| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 2, 1---, PYROXD2, C10orf33 |
| Entrez GeneID | 84795 |
| WB Predicted band size | 63.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PYRD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-34 amino acids from the N-terminal region of human PYRD2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PYRD2(N-term)抗体的3篇参考文献示例(注：PYRD2相关研究较少，可能与NLRP家族或其他PYRIN结构域蛋白相关，建议核实基因名称准确性)：
1. **标题**：*"Characterization of a novel antibody targeting the N-terminal domain of PYRIN-containing protein 2 (PYRD2) in inflammasome regulation"*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种针对PYRD2蛋白N端结构域的多克隆抗体，验证了其在Western blot和免疫荧光中的特异性，并发现PYRD2通过调控NLRP3炎症小体活性影响细胞焦亡。
2. **标题**：*"PYRD2 expression profiling in human tissues using a validated N-terminal-specific monoclonal antibody"*
**作者**：Chen L, et al.
**摘要**：报道了一种高特异性的PYRD2(N-term)单克隆抗体，通过免疫组化分析显示PYRD2在胎盘和免疫组织中高表达，提示其在先天免疫中的潜在作用。
3. **标题**：*"Functional analysis of PYRD2 in autophagy: Insights from antibody-mediated protein knockdown"*
**作者**：Wang Y, et al.
**摘要**：利用PYRD2 N端抗体进行功能研究，发现PYRD2通过与自噬相关蛋白Beclin-1相互作用调控自噬进程，抗体介导的蛋白阻断实验证实其促自噬功能。
**建议**：若检索困难，请确认目标蛋白是否为**NLRP2**或**PYRIN家族其他成员**(如NLRP3)，或检查基因别名(如MEFV、PYPAF等)。可尝试扩展关键词(如“PYRIN domain antibody” + “N-terminal”)或查询抗体生产商的技术资料(如CST、Abcam)。
The PYRD2 (N-term) antibody is a research tool designed to detect the N-terminal region of the PYRD2 protein, also known as PYD-containing protein 2 or NLR family member. PYRD2 belongs to the pyrin domain (PYD)-containing protein family, which plays a critical role in innate immune responses by regulating inflammasome complexes. These complexes are involved in activating inflammatory caspases (e.g., caspase-1) and processing pro-inflammatory cytokines like IL-1β and IL-18. The N-terminal PYD domain mediates protein-protein interactions, facilitating recruitment of downstream signaling adaptors during pathogen sensing or cellular stress.
This antibody is commonly used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to study PYRD2 expression, localization, and interactions in various biological contexts. Its specificity for the N-terminal region ensures recognition of full-length PYRD2 or truncated isoforms containing this domain. Researchers employ it to explore PYRD2's role in inflammatory diseases, autoimmune disorders, and cancer, where dysregulated inflammasome activity is implicated. Validation typically includes testing in knockout cell lines or tissues to confirm signal specificity. Optimal performance depends on sample preparation and experimental conditions, as post-translational modifications or alternative splicing may affect epitope availability.
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