关键信息
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基因名
CDH1
- 应用
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别名
CDH1；CDH1；FYR；FZR；Fizzy-related Protein homolog
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P12830
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表达区间
155-709aa
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氨基酸序列
DWVIPPISCPENEKGPFPKNLVQIKSNKDKEGKVFYSITGQGADTPPVGVFIIERETGWLKVTEPLDRERIATYTLFSHAVSSNGNAVEDPMEILITVTDQNDNKPEFTQEVFKGSVMEGALPGTSVMEVTATDADDDVNTYNAAIAYTILSQDPELPDKNMFTINRNTGVISVVTTGLDRESFPTYTLVVQAADLQGEGLSTTATAVITVTDTNDNPPIFNPTTYKGQVPENEANVVITTLKVTDADAPNTPAWEAVYTILNDDGGQFVVTTNPVNNDGILKTAKGLDFEAKQQYILHVAVTNVVPFEVSLTTSTATVTVDVLDVNEAPIFVPPEKRVEVSEDFGVGQEITSYTAQEPDTFMEQKITYRIWRDTANWLEINPDTGAISTRAELDREDFEHVKNSTYTALIIATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIIDADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKINLKLMDNQNKDQVTTLEVSVCDCEGAAGVCRKAQPVEAGLQIPA
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分子量
61.9 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
CDH1, or cadherin-1, is a crucial cell adhesion molecule primarily expressed in epithelial tissues, playing a significant role in maintaining tissue integrity and regulating cellular interactions. Its impairment is associated with various malignancies, particularly hereditary diffuse gastric cancer (HDGC), linked to germline mutations in the CDH1 gene. Furthermore, CDH1 is involved in the E-cadherin-mediated signaling pathways that control cell proliferation, differentiation, and apoptosis. Research on CDH1 recombinant proteins aims to elucidate their structure-function relationships, facilitating a deeper understanding of how mutations contribute to cancer progression. By producing recombinant CDH1, scientists can investigate its binding affinities and characterize the impact of specific mutations on its adhesive properties. This knowledge is crucial for developing targeted therapies and diagnostic tools for CDH1-related cancers. The study of CDH1 also extends to regenerative medicine and tissue engineering, where promoting proper cell adhesion is essential for successful graft integration and wound healing. Overall, the exploration of CDH1 recombinant proteins remains a vital area of research with promising implications for cancer biology, genetic counseling, and therapeutic strategies.
