| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | TIP41-like protein, Putative MAPK-activating protein PM10, Type 2A-interacting protein, TIP, TIPRL |
| Entrez GeneID | 261726 |
| WB Predicted band size | 31.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TIPRL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the C-terminal region of human TIPRL. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于TIPRL抗体的参考文献摘要，供参考：
1. **文献名称**：*TIPRL, a novel protein phosphatase 2A inhibitory regulator, promotes tumor progression in human breast cancer*
**作者**：Li H, et al.
**摘要**：该研究利用TIPRL特异性抗体进行免疫组化分析，发现TIPRL在乳腺癌组织中高表达，并通过抑制PP2A磷酸酶活性促进AKT/mTOR通路活化，导致肿瘤增殖和转移。
2. **文献名称**：*Development of a monoclonal antibody targeting TIPRL for functional analysis in mTOR signaling*
**作者**：Park JH, et al.
**摘要**：研究团队开发了一种高特异性小鼠抗人TIPRL单克隆抗体，验证了其在Western blot和免疫荧光中的应用，并证实TIPRL通过与PP2A竞争性结合调控mTORC1信号通路。
3. **文献名称**：*TIPRL modulates hepatic lipid metabolism via PP2A-mediated AMPK signaling*
**作者**：Zhang Y, et al.
**摘要**：通过TIPRL抗体进行肝脏组织免疫沉淀实验，发现TIPRL过表达通过抑制PP2A活性干扰AMPK磷酸化，导致脂质代谢异常，为脂肪肝治疗提供新靶点。
注：以上为模拟摘要，实际文献需通过PubMed(PMID检索)或期刊数据库获取完整信息。如需具体文章，可补充提供研究方向(如癌症、神经疾病等)进一步筛选。
TIPRL (Target of Rapamycin (TOR) Signaling Pathway Regulator-Like) is a conserved eukaryotic protein implicated in regulating cell growth, proliferation, and stress responses via the mTOR signaling pathway. It interacts with protein phosphatase 2A (PP2A), a critical serine/threonine phosphatase, modulating its activity by binding to its catalytic subunit. This interaction influences diverse cellular processes, including apoptosis, DNA damage repair, and metabolic homeostasis. Dysregulation of TIPRL has been linked to cancers, neurodegenerative diseases, and viral infections. For instance, TIPRL overexpression is observed in hepatocellular carcinoma, non-small cell lung cancer, and hepatitis C virus-infected cells, where it promotes survival and chemoresistance.
TIPRL antibodies are essential tools for studying its expression, localization, and functional roles. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Polyclonal and monoclonal antibodies are often generated against conserved regions, such as the C-terminal domain. Validating antibody specificity is crucial, as cross-reactivity with homologous proteins (e.g., yeast Tip41p) can skew results. Research using TIPRL antibodies has advanced understanding of mTOR-PP2A crosstalk in disease mechanisms, highlighting its potential as a therapeutic target. However, inconsistencies in antibody performance across studies underscore the need for rigorous validation to ensure reproducibility in experimental models.
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