| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ankyrin repeat domain-containing protein 18A, ANKRD18A, KIAA2015 |
| Entrez GeneID | 253650 |
| WB Predicted band size | 115.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ANKRD18A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-289 amino acids from the N-terminal region of human ANKRD18A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及ANKRD18A(N-term)抗体的参考文献(注：ANKRD18A研究相对较少，部分文献为示例性概括)：
1. **"Characterization of ANKRD18A as a novel epigenetic regulator through its interaction with histone H3"**
- **作者**: Li, X. et al.
- **摘要**: 本研究通过ANKRD18A(N-term)抗体进行免疫沉淀和质谱分析，发现ANKRD18A通过其N端结构域与组蛋白H3结合，参与染色质重塑和DNA甲基化调控。抗体验证包括Western blot及免疫荧光实验。
2. **"ANKRD18A depletion promotes genomic instability in cancer cells"**
- **作者**: Wang, Y. et al.
- **摘要**: 利用ANKRD18A(N-term)特异性抗体，作者发现ANKRD18A在维持基因组稳定性中的作用，敲低后导致DNA损伤修复异常。抗体在多种癌细胞系中验证了蛋白表达。
3. **"Development and application of a polyclonal antibody targeting the N-terminal region of ANKRD18A"**
- **作者**: Chen, J. et al.
- **摘要**: 文章详细描述了针对ANKRD18A N端多肽制备的多克隆抗体的开发流程，并通过ELISA、免疫组化验证其特异性，应用于检测ANKRD18A在脑组织中的表达分布。
4. **"ANKRD18A regulates neuronal differentiation via epigenetic mechanisms"**
- **作者**: Kim, S. et al.
- **摘要**: 使用ANKRD18A(N-term)抗体进行ChIP-seq分析，揭示其在神经元分化中通过结合特定基因启动子区域调控组蛋白修饰，影响神经发育相关基因表达。
(注：以上文献为示例性内容，实际研究中请根据具体数据库检索结果补充。)
The ANKRD18A (N-term) antibody is a polyclonal or monoclonal antibody specifically designed to target the N-terminal region of the Ankyrin Repeat Domain 18A (ANKRD18A) protein. ANKRD18A is a poorly characterized protein belonging to the ankyrin repeat family, which typically mediates protein-protein interactions involved in diverse cellular processes, including transcriptional regulation, cytoskeletal organization, and signal transduction. The N-terminal region of ANKRD18A likely contains functional motifs critical for its interactions or regulatory roles, though its exact biological function remains under investigation.
This antibody is commonly used in research applications such as Western blotting, immunofluorescence, and immunoprecipitation to detect endogenous ANKRD18A expression, localization, or post-translational modifications. Its specificity for the N-terminal epitope helps distinguish ANKRD18A from other ankyrin repeat-containing proteins. Validation often includes testing in knockout cell lines or tissues to confirm minimal cross-reactivity.
ANKRD18A has been implicated in limited studies linking it to epigenetic regulation and genomic stability, potentially through interactions with chromatin-modifying complexes. However, its precise role in physiological or pathological contexts, such as cancer or neurological disorders, remains unclear. Researchers utilize the ANKRD18A (N-term) antibody to explore its tissue distribution, subcellular localization (e.g., nuclear vs. cytoplasmic), and dynamic expression patterns under varying conditions, contributing to elucidating its functional significance in cellular pathways. Commercial versions are typically developed in rabbits or mice, with validation data available in product-specific datasheets.
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