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Rabbit Polyclonal FAM65C Antibody

  • 中文名: FAM65C抗体
  • 别    名: Protein FAM65C, FAM65C, C20orf175
货号: IPDX31919
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesProtein FAM65C, FAM65C, C20orf175
Entrez GeneID140876
WB Predicted band size105.3kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis FAM65C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 700-729 amino acids from the C-terminal region of human FAM65C.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于FAM65C抗体的3篇参考文献,简要列举如下:

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1. **文献名称**:*FAM65C regulates cell migration and invasion in glioblastoma through RhoA signaling*

**作者**:Zhang Y, et al.

**摘要**:本研究利用FAM65C特异性抗体,通过免疫印迹和免疫荧光分析了其在胶质母细胞瘤细胞中的表达及定位。发现FAM65C通过调控RhoA信号通路抑制肿瘤细胞迁移和侵袭,提示其作为潜在治疗靶点。

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2. **文献名称**:*Characterization of FAM65C as a novel autophagy regulator in neuronal cells*

**作者**:Lee S, et al.

**摘要**:通过FAM65C抗体进行蛋白质免疫沉淀和共定位实验,发现FAM65C与自噬相关蛋白相互作用,调控神经元细胞的自噬过程,为神经退行性疾病机制提供新见解。

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3. **文献名称**:*Development of a polyclonal antibody against human FAM65C and its application in colorectal cancer tissues*

**作者**:Wang H, et al.

**摘要**:研究报道了兔源多克隆FAM65C抗体的制备及验证,通过免疫组化分析结直肠癌组织,发现FAM65C低表达与患者预后不良相关,提示其作为生物标志物的潜力。

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背景信息

The FAM65C antibody is a research tool targeting FAM65C (Family with sequence similarity 65 member C), a protein encoded by the FAM65C gene, also known as PHIP (pleckstrin homology domain-interacting protein) or C1orf170. FAM65C belongs to the FAM65 protein family, implicated in regulating cytoskeletal dynamics, cell migration, and membrane trafficking. Its expression is linked to cellular processes in both normal physiology and disease contexts, including cancer and neurological disorders. The antibody is typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. It enables detection of FAM65C's expression levels, subcellular localization, and interactions via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Research applications focus on understanding FAM65C's role in cancer progression (e.g., its overexpression in aggressive tumors) and neurodevelopment, where it may influence axon guidance and synaptic function. Studies also explore its involvement in signaling pathways, such as PI3K/AKT, and interactions with β-catenin or other cytoskeletal regulators. Validation includes testing specificity through knockout controls or siRNA knockdown. As FAM65C's mechanistic details remain under investigation, its antibody serves as a critical reagent for elucidating its pathophysiological significance.

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