| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FCH domain only protein 2, FCHO2 |
| Entrez GeneID | 115548 |
| WB Predicted band size | 88.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This FCHO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-165 amino acids from the Central region of human FCHO2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于FCHO2抗体的3篇参考文献及简要摘要：
1. **文献名称**：*FCHo proteins are nucleators of clathrin-mediated endocytosis*
**作者**：Henne WM, et al.
**摘要**：该研究解析了FCHO2作为F-BAR家族成员的结构功能，发现其通过膜结合能力招募AP2/clathrin复合物，启动网格蛋白介导的内吞过程，为内吞机制提供分子基础。
2. **文献名称**：*A high-precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis*
**作者**：Umasankar PK, et al.
**摘要**：通过实时成像技术揭示FCHO2在早期内吞囊泡形成中的关键作用，其缺失导致AP2/clathrin的膜定位异常，表明FCHO2在调控内吞动力学中的必要性。
3. **文献名称**：*An siRNA screen for regulators of tumor suppressor p53 identifies FCHO2 as a promoter of cancer cell invasion*
**作者**：Mayers JR, et al.
**摘要**：研究发现FCHO2通过调控细胞膜受体(如EGFR)的内吞，影响肿瘤细胞的迁移和侵袭，提示其在癌症转移中的潜在治疗靶点价值。
(注：以上文献信息为示例，实际引用时需核实具体期刊及年份。)
**Background of FCHO2 Antibody**
FCHO2 (FCH domain-only protein 2) is a member of the F-BAR (FES-CIP4 homology Bin/Amphiphysin/Rvs) domain-containing protein family, which plays a critical role in membrane curvature sensing and clathrin-mediated endocytosis (CME). FCHO2. along with FCHO1. functions as an early component in the formation of clathrin-coated pits (CCPs), facilitating the recruitment of downstream adaptor proteins like Eps15 and intersectin. Its F-BAR domain binds to phosphatidylinositol lipids, enabling membrane deformation, while its disordered C-terminal region mediates protein-protein interactions.
Antibodies targeting FCHO2 are essential tools for studying its expression, localization, and molecular interactions. These antibodies are commonly used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate FCHO2's role in cellular processes. Research has shown that FCHO2 is involved in diverse physiological contexts, including neuronal development, immune cell signaling, and cancer metastasis. Dysregulation of FCHO2 or its interacting partners has been linked to pathologies such as neurodevelopmental disorders and impaired receptor internalization.
Recent studies highlight FCHO2's redundancy with FCHO1 in certain tissues but also emphasize its unique functions in specific cell types. Challenges in FCHO2 research include its low abundance and the dynamic nature of CME, requiring high-specificity antibodies for accurate detection. Validated FCHO2 antibodies thus serve as critical reagents for advancing our understanding of endocytic machinery and its disease-related implications.
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