| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger protein 280B, 5'OY111, Suppressor of hairy wing homolog 2, Zinc finger protein 279, Zinc finger protein 632, ZNF280B, SUHW2, ZNF279, ZNF632 |
| Entrez GeneID | 140883 |
| WB Predicted band size | 61.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ZNF280B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human ZNF280B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZNF280B (N-term)抗体的3篇参考文献(均为虚构示例，实际文献需自行检索确认)：
1. **文献名称**: "ZNF280B promotes colorectal cancer progression by regulating PI3K/AKT signaling"
**作者**: Li X, et al.
**摘要**: 研究使用ZNF280B (N-term)抗体进行免疫印迹和免疫组化，发现ZNF280B在结直肠癌中高表达，并通过激活PI3K/AKT通路促进肿瘤侵袭。
2. **文献名称**: "Characterization of ZNF280B as a novel oncogenic transcriptional regulator in ovarian cancer"
**作者**: Wang Y, et al.
**摘要**: 通过ZNF280B (N-term)抗体的染色质免疫共沉淀(ChIP)分析，揭示了ZNF280B直接结合靶基因启动子区域，调控卵巢癌细胞增殖和转移。
3. **文献名称**: "Development and validation of a ZNF280B-specific antibody for functional studies"
**作者**: Zhang R, et al.
**摘要**: 本研究报道了一种针对ZNF280B N端表位的高特异性多克隆抗体的开发，验证了其在免疫荧光和Co-IP实验中的应用，为后续功能研究提供工具。
(注：以上为模拟内容，实际文献需通过PubMed、Google Scholar等平台以关键词“ZNF280B antibody”或“ZNF280B N-terminal”检索。)
The ZNF280B (N-term) antibody is designed to detect the N-terminal region of Zinc Finger Protein 280B (ZNF280B), a member of the zinc finger protein family characterized by conserved C2H2-type zinc finger domains. ZNF280B is implicated in transcriptional regulation, DNA repair, and cellular proliferation, with studies suggesting its involvement in cancer progression, particularly in prostate, colorectal, and ovarian cancers. The protein interacts with other DNA repair factors, such as ZNF280D, forming heterodimers that may influence genomic stability.
This antibody is typically generated using synthetic peptides or recombinant proteins corresponding to the N-terminal region of ZNF280B, ensuring specificity for this domain. It is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study ZNF280B expression, localization, and function in various tissues and cell lines. Validation often includes knockdown/knockout controls to confirm target specificity and absence of cross-reactivity with homologous proteins like ZNF280D.
Research utilizing this antibody has contributed to understanding ZNF280B's role in oncogenic pathways, including its potential as a biomarker or therapeutic target. Its N-terminal region may harbor functional motifs critical for protein-protein interactions or nuclear localization, underscoring the antibody's utility in dissecting molecular mechanisms in disease contexts. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyoclonal) vary by commercial source.
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