| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transmembrane channel-like protein 4, TMC4 |
| Entrez GeneID | 147798 |
| WB Predicted band size | 79.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TMC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-94 amino acids from the N-terminal region of human TMC4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TMC4(N-term)抗体的3-4篇虚构参考文献示例(实际文献需根据具体研究补充)：
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**1. 文献名称**：Structural and functional characterization of the TMC4 N-terminal domain in ion channel regulation
**作者**：Smith A, et al.
**摘要**：本研究利用TMC4(N-term)特异性抗体，通过免疫沉淀和冷冻电镜技术解析了TMC4蛋白N端结构域的构象，揭示了其在钙离子通道调控中的关键作用，并证明该抗体可有效用于Western blot和免疫荧光检测。
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**2. 文献名称**：TMC4 expression profiling in gastrointestinal tissues using a novel N-terminal specific antibody
**作者**：Zhang L, et al.
**摘要**：作者开发了一种针对TMC4 N端的高特异性多克隆抗体，通过免疫组化分析发现TMC4在结肠上皮细胞中高表达，提示其可能参与肠道离子转运及液体分泌的病理生理过程。
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**3. 文献名称**：Role of TMC4 in auditory hair cells: Insights from antibody-mediated knockdown experiments
**作者**：Johnson R, et al.
**摘要**：研究利用TMC4(N-term)抗体进行内耳毛细胞的定位分析，发现TMC4定位于纤毛基部，并通过抗体阻断实验证实其与遗传性听力障碍的相关性，为耳聋机制提供了新见解。
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**4. 文献名称**：Development and validation of a TMC4 N-terminal monoclonal antibody for cancer biomarker screening
**作者**：Chen H, et al.
**摘要**：该研究报道了一种新型TMC4 N端单克隆抗体的开发，验证其在ELISA和流式细胞术中的高灵敏度和特异性，并发现TMC4在部分乳腺癌组织中异常高表达，可能作为潜在生物标志物。
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**注**：以上文献为示例，实际引用需根据真实研究调整。建议通过PubMed、Google Scholar等平台以“TMC4 antibody”或“TMC4 N-terminal”为关键词检索最新文献。
The TMC4 (N-term) antibody is designed to target the N-terminal region of the transmembrane channel-like 4 (TMC4) protein, a member of the TMC family implicated in ion transport and cellular signaling. TMC proteins are evolutionarily conserved and share structural features, including multiple transmembrane domains. While TMC1 and TMC2 are well-studied for their roles in auditory function, TMC4 remains less characterized, though it is hypothesized to participate in calcium or sodium ion homeostasis, potentially influencing epithelial or secretory processes.
The TMC4 (N-term) antibody is typically produced in rabbits using synthetic peptides or recombinant proteins corresponding to the N-terminal sequence of human TMC4. It is commonly validated for applications such as Western blotting, immunofluorescence, and immunohistochemistry to detect endogenous TMC4 expression in tissues or cell lines. Researchers employ this antibody to investigate TMC4's localization, expression patterns, and interaction partners, particularly in studies exploring its physiological roles in organs like the kidney, liver, or intestines, where ion transport is critical.
Due to the limited understanding of TMC4's precise function, this antibody serves as a key tool for elucidating its contributions to cellular pathways, disease mechanisms (e.g., metabolic disorders), or its potential as a therapeutic target. Specificity is often confirmed via knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity with other TMC family members.
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