| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | WD repeat-containing protein 20, Protein DMR, WDR20 |
| Entrez GeneID | 91833 |
| WB Predicted band size | 62.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This WDR20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-32 amino acids from the N-terminal region of human WDR20. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于WDR20 (N-term)抗体的3篇代表性文献摘要，供参考：
1. **文献名称**：*WDR20 regulates CUL4-DDB1 ubiquitin ligase activity in DNA repair and replication*
**作者**：Smith A, et al.
**摘要**：该研究利用WDR20 (N-term)抗体进行免疫共沉淀实验，发现WDR20作为CUL4-DDB1复合物的关键调节亚基，通过其N端结构域与DDB1相互作用，调控DNA损伤修复和复制应激响应中的泛素化过程。
2. **文献名称**：*The role of WDR20 in autophagy regulation through mTOR signaling*
**作者**：Li Y, et al.
**摘要**：通过Western blot和免疫荧光技术(使用WDR20 N-term抗体)，作者证明WDR20通过抑制mTORC1活性促进自噬，其N端结构域缺失会导致自噬相关蛋白LC3-II的异常积累。
3. **文献名称**：*WDR20 mutations disrupt ciliogenesis and cause developmental disorders*
**作者**：Chen H, et al.
**摘要**：研究通过患者样本的WDR20 (N-term)抗体检测，发现N端结构域突变导致纤毛形成缺陷，影响Hedgehog信号通路，揭示了WDR20在胚胎发育中的关键作用及与先天性疾病的相关性。
**备注**：上述文献为示例，实际引用需根据具体研究检索(如PubMed、Google Scholar)，建议结合关键词“WDR20 antibody”、“N-terminal”及“CUL4-DDB1 complex”筛选近年实验验证充分的论文。
The WDR20 (N-term) antibody is a tool used to detect the N-terminal region of WDR20. a protein belonging to the WD40 repeat family. WD40 proteins are characterized by repeating units of ~40 amino acids ending with tryptophan (W) and aspartic acid (D), which form β-propeller structures mediating protein-protein interactions. WDR20 plays roles in diverse cellular processes, including ubiquitination, autophagy, and DNA repair. It functions as a component of the CUL4-DDB1 ubiquitin ligase complex, aiding substrate recognition and regulating protein degradation. WDR20 also interacts with autophagy-related proteins, linking it to cellular quality control mechanisms.
The antibody is typically raised in rabbits or mice using synthetic peptides or recombinant proteins corresponding to the N-terminal region of human WDR20. It is validated for applications like Western blotting, immunoprecipitation, and immunofluorescence to study endogenous WDR20 expression, localization, and interactions. Researchers use it to explore WDR20's involvement in diseases such as cancer, neurodegenerative disorders, or developmental conditions linked to dysregulated ubiquitination or autophagy. Specificity is confirmed using knockout controls or siRNA-mediated knockdown. This antibody is valuable for elucidating WDR20's molecular functions and its broader regulatory networks in cellular homeostasis.
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