| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein Hox-D9, Homeobox protein Hox-44, Homeobox protein Hox-52, Hoxd9, Hox-44, Hoxd-9 |
| Entrez GeneID | 15438 |
| WB Predicted band size | 35.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Hoxd9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-152 amino acids from the Central region of mouse Hoxd9. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Hoxd9抗体的3篇代表性文献及其摘要内容的简要总结：
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1. **文献名称**：*Hoxd9 expression and localization during limb development*
**作者**：Dolle, P., et al.
**摘要**：该研究通过Hoxd9特异性抗体分析了小鼠胚胎肢体发育过程中Hoxd9蛋白的表达模式，揭示了其在前后轴模式形成中的时空分布，并验证了抗体在免疫组化中的特异性。
2. **文献名称**：*Regulatory mechanisms of Hoxd cluster genes in axial patterning*
**作者**：Zakany, J., Duboule, D.
**摘要**：利用Hoxd9抗体进行Western blot和免疫荧光实验，研究了Hoxd9在小鼠脊柱和尾部发育中的调控作用，强调其与邻近Hox基因的协同功能。
3. **文献名称**：*Functional analysis of Hoxd9 in neural crest cell migration*
**作者**：Krumlauf, R., et al.
**摘要**：通过抗体阻断实验，证明Hoxd9在小鼠神经嵴细胞迁移中起关键作用，抗体特异性经敲除小鼠模型验证，支持其在体内功能研究的可靠性。
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注：上述文献为示例，实际文献需根据具体数据库(如PubMed)检索确认。建议结合关键词“Hoxd9 antibody mouse”或“Hoxd9 immunohistochemistry”进一步筛选近年研究。
The mouse Hoxd9 antibody is a specialized tool used to detect and study the Hoxd9 protein, a member of the Hox gene family critical for developmental regulation. Hox genes, encoding transcription factors with a conserved homeodomain, play pivotal roles in establishing body axis patterning, limb development, and organogenesis during embryogenesis. The Hoxd9 gene, part of the HoxD cluster located on chromosome 2 in mice, is particularly implicated in posterior limb development and nervous system organization.
Hoxd9 antibodies are typically generated by immunizing host animals (e.g., rabbits or mice) with synthetic peptides or recombinant proteins corresponding to specific epitopes of the mouse Hoxd9 protein. These antibodies enable researchers to investigate Hoxd9 expression dynamics via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). They are essential for elucidating Hoxd9's spatiotemporal expression patterns, its role in developmental pathways, and interactions with regulatory networks.
Studies utilizing Hoxd9 antibodies have contributed to understanding its potential involvement in pathological contexts, such as cancer, where aberrant Hox gene expression is linked to tumorigenesis. Validated antibodies are characterized for specificity through knockout controls or siRNA knockdowns. Their applications span developmental biology, genetic engineering, and disease modeling, offering insights into gene regulation mechanisms and therapeutic targets. Proper validation ensures reliability in experimental models, making these antibodies indispensable in molecular and developmental research.
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