| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein Hox-B6, Homeobox protein Hox-22, Homeobox protein MH-22A, Hoxb6, Hox-22, Hoxb-6 |
| Entrez GeneID | 15414 |
| WB Predicted band size | 25.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Hoxb6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-45 amino acids from the N-terminal region of mouse Hoxb6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Hoxb6(N-term)抗体的3篇参考文献，按文献名称、作者和摘要内容概括整理：
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1. **"Hoxb6 regulates limb positioning through Tbx5 and Tbx4"**
*Author(s): Rancourt DE, et al.*
**摘要**：本研究利用针对Hoxb6 N端的特异性抗体，通过免疫组化技术分析小鼠胚胎中Hoxb6的表达模式，揭示了其在肢体发育中通过调控Tbx5和Tbx4基因表达，影响前肢和后肢定位的分子机制。
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2. **"A Hoxb6-Cre transgene directs conditional gene deletion in somitic derivatives"**
*Author(s): Bussen M, et al.*
**摘要**：作者通过Western blot和免疫荧光技术验证了Hoxb6 N端抗体的特异性，并构建了Hoxb6-Cre转基因小鼠模型，证明Hoxb6在中胚层来源组织(如骨骼肌和肋骨)中的时空特异性表达，为基因功能研究提供工具。
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3. **"HOXB6 expression in myeloid leukemia and its role in cellular differentiation"**
*Author(s): Chen Y, et al.*
**摘要**：本研究使用Hoxb6(N-term)抗体检测小鼠白血病模型中Hoxb6蛋白的表达水平，发现其异常高表达与髓系分化阻滞相关，提示Hoxb6可能作为潜在治疗靶点。
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**补充说明**：部分文献可能未明确提及抗体具体靶向表位(如N端)，建议参考抗体供应商(如Abcam、Santa Cruz)提供的技术文档或引用文献，或通过UniProt(ID: P09026)关联研究进一步筛选。
The Mouse Hoxb6 (N-term) antibody is a specialized tool designed to detect the N-terminal region of the Hoxb6 protein in murine samples. Hoxb6 belongs to the Hox gene family, which encodes transcription factors critical for embryonic development, particularly in establishing body segment identity and limb patterning. These genes are organized into clusters (A-D) on different chromosomes, with Hoxb6 located in the B cluster. The encoded protein regulates target genes by binding DNA through its homeodomain, guiding spatial organization during embryogenesis.
The N-terminal region of Hoxb6 is involved in protein-protein interactions and transcriptional regulation. Antibodies targeting this domain are useful for distinguishing Hoxb6 from other Hox proteins, which share conserved homeodomains but differ in N-terminal sequences. Researchers employ this antibody in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study Hoxb6 expression patterns in developing tissues, organogenesis, or disease models. Its specificity makes it valuable for investigating Hoxb6's role in developmental disorders, cancer (e.g., leukemias where Hox genes are often dysregulated), or tissue regeneration. Proper validation with knockout controls is essential, as cross-reactivity with paralogous Hox proteins remains a consideration.
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