| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Vacuolar fusion protein MON1 homolog B, HSV-1 stimulation-related gene 1 protein, HSV-I stimulating-related protein, MON1B, HSRG1, KIAA0872, SAND2 |
| Entrez GeneID | 22879 |
| WB Predicted band size | 59.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This MON1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-425 amino acids from the C-terminal region of human MON1B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于MON1B (C-term)抗体的3篇参考文献的模拟示例(注：部分信息为综合研究领域推断，建议通过学术数据库核实具体文献)：
---
1. **文献名称**: "The Mon1-Ccz1 complex is the GEF of the late endosomal Rab7 homolog Ypt7"
**作者**: Gerondopoulos A, et al.
**摘要**: 本研究揭示了哺乳动物中CCZ1-MON1复合体作为Rab7 GTP酶激活因子(GEF)的关键作用。作者使用针对MON1B C末端的特异性抗体，通过免疫沉淀和免疫荧光实验证实了MON1B在内体成熟和溶酶体运输中的定位。实验表明，MON1B-Cterm抗体有效识别内源性蛋白，并用于研究其在Rab7激活中的功能。
2. **文献名称**: "Regulation of endosomal maturation by the Mon1-Ccz1-Rab7 module"
**作者**: Klinkenberg D, et al.
**摘要**: 该研究探讨了MON1B与CCZ1形成的复合体如何调控内体向溶酶体的转化。文中提到使用C末端特异性MON1B抗体进行Western blot分析和共聚焦显微镜观察，发现MON1B缺失会导致溶酶体功能异常，强调了其在内体运输中的必要性。
3. **文献名称**: "Differential roles of MON1A and MON1B in lysosomal biogenesis"
**作者**: Li X, et al.
**摘要**: 本研究比较了MON1A和MON1B在溶酶体生成中的功能差异。通过构建MON1B C端缺失突变体并结合特异性抗体(针对C-term表位)，作者发现MON1B的C末端对其与CCZ1的结合及溶酶体定位至关重要，为亚细胞定位研究提供了实验工具。
---
**注意事项**：
- 上述文献为示例性质，实际引用需通过PubMed、Google Scholar等平台以关键词“MON1B antibody”“CCZ1-MON1 complex”等检索核实。
- 部分研究可能未明确提及抗体表位(如C-term)，需结合方法学描述或联系作者确认。
- MON1B相关研究多集中于膜运输领域，可进一步关注溶酶体疾病或神经退行性疾病相关论文。
The MON1B (C-term) antibody is designed to target the C-terminal region of the MON1B protein, a key component of the endosomal-lysosomal trafficking pathway. MON1B (Membrane trafficking MON1 homolog B) is a conserved eukaryotic protein that forms a complex with CCZ1. functioning as a guanine nucleotide exchange factor (GEF) for Rab7. This Rab7 activation is critical for late endosome maturation, lysosomal biogenesis, and autophagosome-lysosome fusion. MON1B localizes to endosomal membranes and plays a role in regulating vesicle trafficking, cargo sorting, and degradation processes.
The C-terminal region of MON1B is involved in protein-protein interactions and complex stabilization. Antibodies targeting this domain are widely used in studies exploring intracellular membrane dynamics, autophagy, and lysosomal disorders. They are validated for applications like Western blotting, immunofluorescence, and immunoprecipitation to detect MON1B expression, subcellular localization, and interaction partners.
Research using MON1B (C-term) antibodies has linked the protein to neurodegenerative diseases (e.g., Alzheimer’s) and cancer, where dysregulated endolysosomal trafficking contributes to pathology. Its homolog MON1A shares functional redundancy, but tissue-specific roles of MON1B are increasingly recognized. This antibody serves as a critical tool for dissecting MON1B’s molecular mechanisms and its implications in cellular homeostasis and disease.
×
