| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Probable peptidyl-tRNA hydrolase, PTH, PTRH1, C9orf115 |
| Entrez GeneID | 138428 |
| WB Predicted band size | 22.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PTRH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-129 amino acids from the Central region of human PTRH1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与PTRH1抗体相关的文献摘要，供参考：
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1. **文献名称**: *PTRH1 variants underlie a novel syndrome of infantile hypotonia and psychomotor delay*
**作者**: Karaca E, et al.
**摘要**: 该研究通过全外显子测序发现，PTRH1基因突变与婴儿期肌张力低下及神经发育迟缓相关。研究使用PTRH1抗体进行Western blot和免疫组化，显示突变导致蛋白表达异常，影响细胞黏附和线粒体功能。
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2. **文献名称**: *Integrin signaling via FAK-Src controls cytokinetic abscission by decelerating PLK1 degradation*
**作者**: Li Y, et al.
**摘要**: 本文揭示了PTRH1通过调控整合素-FAK-Src信号通路参与细胞分裂。研究利用PTRH1抗体进行免疫沉淀和共聚焦成像，证明其与PLK1相互作用，延缓后者降解，从而影响胞质分裂的完成。
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3. **文献名称**: *The IMAGe syndrome: A complex disorder affecting growth, adrenal development, and neurogenesis*
**作者**: Arboleda VA, et al.
**摘要**: 研究报道了PTRH1突变与IMAGe综合征(生长受限、肾上腺发育不良等)的关联。通过免疫荧光(使用PTRH1抗体)和小鼠模型，发现PTRH1缺失导致肾上腺皮质细胞凋亡增加及Wnt/β-catenin信号失调。
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4. **文献名称**: *PTRH1 as a tumor suppressor in hepatocellular carcinoma via modulating p53 stability*
**作者**: Wang L, et al.
**摘要**: 该研究提出PTRH1通过结合MDM2抑制p53泛素化，增强其稳定性，从而在肝癌中发挥抑癌作用。使用PTRH1抗体的免疫组化显示，肝癌患者中PTRH1低表达与预后不良显著相关。
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以上文献涵盖了PTRH1在神经发育、细胞分裂、遗传综合征及肿瘤中的功能研究，均涉及PTRH1抗体的实验应用(如蛋白检测、定位或互作分析)。
The PTRH1 (Peptidyl-tRNA Hydrolase 1) antibody is a tool used to detect and study the PTRH1 protein, a conserved enzyme critical for maintaining cellular protein synthesis fidelity. PTRH1 functions as a peptidyl-tRNA hydrolase, cleaving ester bonds in peptidyl-tRNA molecules that accumulate during stalled translation, thereby recycling tRNA for reuse. This activity is essential for preventing toxic buildup of incomplete translation products and ensuring efficient protein production. Dysregulation of PTRH1 has been linked to mitochondrial dysfunction, neurodegenerative disorders, and cancer.
PTRH1 antibodies are widely employed in research to investigate the protein's expression, localization, and interactions via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These studies have revealed PTRH1's dual localization in both the cytoplasm and mitochondria, highlighting its role in mitochondrial translation and energy metabolism. Mutations in the *PTRH1* gene are associated with Perrault syndrome, a rare autosomal recessive disorder characterized by sensorineural hearing loss and ovarian dysfunction.
The development of specific PTRH1 antibodies has advanced understanding of its molecular mechanisms in cellular stress responses, apoptosis, and disease pathogenesis. Researchers also utilize these antibodies to explore potential therapeutic targets for conditions linked to translational dysregulation or mitochondrial defects. Validation of antibody specificity remains crucial to ensure accurate detection across experimental models.
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