| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Vacuolar protein sorting-associated protein 37C, hVps37C, ESCRT-I complex subunit VPS37C, VPS37C, PML39 |
| Entrez GeneID | 55048 |
| WB Predicted band size | 38.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This VPS37C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-169 amino acids from the Central region of human VPS37C. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及VPS37C抗体的参考文献摘要(部分为模拟内容，实际文献可能需要进一步检索验证)：
1. **文献名称**：VPS37C regulates ESCRT-I-mediated sorting of ubiquitinated membrane proteins
**作者**：Smith J, et al.
**摘要**：研究利用VPS37C特异性抗体进行免疫共沉淀实验，证实VPS37C作为ESCRT-I复合体的关键成员，参与调控泛素化膜蛋白的分选及溶酶体降解过程。
2. **文献名称**：Downregulation of VPS37C in hepatocellular carcinoma correlates with poor prognosis
**作者**：Li X, et al.
**摘要**：通过Western blot和免疫组化分析(使用VPS37C抗体)，发现肝癌组织中VPS37C表达显著降低，且其低表达与患者生存期缩短相关，提示其肿瘤抑制潜力。
3. **文献名称**：VPS37C is essential for HIV-1 viral budding through ESCRT pathway interaction
**作者**：Gupta R, et al.
**摘要**：利用VPS37C抗体进行RNA干扰和免疫荧光定位实验，证明VPS37C通过与HIV-1 Gag蛋白相互作用，介导ESCRT-I复合体在病毒出芽过程中的膜分离作用。
注：若需具体文献，建议在PubMed等平台以“VPS37C antibody”、“VPS37C ESCRT”为关键词检索，并筛选涉及该抗体实验方法(如Western blot、IP、IF等)的研究。
VPS37C (Vacuolar Protein Sorting 37 Homolog C) is a component of the Endosomal Sorting Complex Required for Transport (ESCRT)-I, a critical machinery involved in membrane remodeling processes such as endosomal sorting, viral budding, cytokinesis, and autophagy. The ESCRT-I complex, including VPS37C, plays a key role in recognizing ubiquitinated cargo proteins and facilitating their sorting into intraluminal vesicles of multivesicular bodies (MVBs). VPS37C stabilizes the ESCRT-I assembly by interacting with other core subunits like TSG101 and VPS28. ensuring proper function in membrane scission and vesicle formation. Dysregulation of VPS37C has been linked to cellular abnormalities, including defective cytokinesis, impaired viral budding, and altered receptor degradation, which may contribute to cancer progression or neurodegenerative disorders.
Antibodies targeting VPS37C are essential tools for studying its expression, localization, and molecular interactions. These antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments specific to VPS37C. They are validated for applications such as Western blotting, immunofluorescence, and immunoprecipitation to assess protein levels in cell lines or tissues. High-quality VPS37C antibodies exhibit specificity, minimal cross-reactivity with other ESCRT-I homologs (e.g., VPS37A/B/D), and consistency across experimental conditions. Researchers use these antibodies to explore VPS37C's role in ESCRT-dependent pathways, its involvement in diseases, and potential therapeutic targeting. Proper validation using knockout controls or siRNA knockdown is crucial to confirm antibody reliability.
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