| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein Nkx-32, Bagpipe homeobox protein homolog 1, Homeobox protein NK-3 homolog B, NKX3-2, BAPX1, NKX3B |
| Entrez GeneID | 579 |
| WB Predicted band size | 34.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NKX3-2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 208-235 amino acids from the Central region of human NKX3-2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及NKX3-2抗体的参考文献摘要：
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1. **文献名称**：*NKX3-2 regulates chondrocyte differentiation during skeletal development*
**作者**：Tribioli C. et al.
**摘要**：该研究通过免疫组化和Western blot分析，利用NKX3-2特异性抗体，揭示了NKX3-2在小鼠胚胎软骨形成中的关键作用，证明其通过调控Sox9等基因参与软骨细胞分化。
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2. **文献名称**：*Aberrant NKX3-2 expression in leukemia and its role in cell proliferation*
**作者**：Okubo T. & Morishima K.
**摘要**：研究使用NKX3-2抗体检测白血病细胞系中的蛋白表达，发现NKX3-2异常高表达与细胞增殖相关，提示其可能作为血液肿瘤的潜在治疗靶点。
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3. **文献名称**：*NKX3-2 mutations cause craniofacial defects via dysregulated osteogenesis*
**作者**：Mundlos S. et al.
**摘要**：通过患者样本和小鼠模型，结合NKX3-2抗体的免疫荧光染色，证实NKX3-2基因突变导致颅面骨发育异常，机制涉及成骨细胞分化的转录调控紊乱。
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注：以上文献为示例性概括，实际引用需核对具体论文来源及细节。
The NKX3-2 antibody is a tool used to detect NKX3-2. a transcription factor encoded by the NKX3-2 gene (also known as Bapx1). This gene belongs to the NKX family of homeodomain-containing proteins, which play critical roles in embryonic development. NKX3-2 is particularly vital in skeletal and chondrogenic patterning, regulating the differentiation of mesenchymal cells into chondrocytes during early development. It is expressed in specific regions of the developing skeleton, including the vertebral column, limbs, and craniofacial structures, and is involved in maintaining progenitor cell populations in cartilage.
Antibodies targeting NKX3-2 are essential for studying its expression, localization, and function in developmental biology and disease models. Researchers use these antibodies in techniques like immunohistochemistry, Western blotting, and immunofluorescence to investigate NKX3-2's role in skeletal disorders, such as spondylo-megaepiphyseal-metaphyseal dysplasia (SMMD), caused by NKX3-2 mutations. The antibody helps identify molecular mechanisms underlying cartilage malformations and potential therapeutic targets. Commercial NKX3-2 antibodies are typically raised in rabbits or mice, with validation in knockout models to ensure specificity. Their application extends to developmental studies, cancer research (e.g., leukemia), and regenerative medicine, where understanding chondrocyte regulation is crucial for tissue engineering. Reliable NKX3-2 antibodies thus serve as a cornerstone in both basic and translational research.
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