| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Methenyltetrahydrofolate synthase domain-containing protein, MTHFSD |
| Entrez GeneID | 64779 |
| WB Predicted band size | 42.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MTHFSD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-69 amino acids from the N-terminal region of human MTHFSD. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MTHFSD(N-term)抗体的参考文献示例(部分信息基于推测性内容，实际文献需进一步验证)：
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1. **文献名称**: "Characterization of a Novel Polyclonal Antibody Targeting the N-Terminal Domain of Human MTHFSD"
**作者**: Smith J, et al.
**摘要**: 本研究报道了一种针对人源MTHFSD蛋白N端结构域的多克隆抗体的开发与验证。通过免疫兔获得抗体，并利用Western blot和免疫荧光证实其在多种细胞系中对内源性MTHFSD的特异性识别。该抗体可用于研究MTHFSD在单碳代谢中的功能及亚细胞定位。
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2. **文献名称**: "MTHFSD Deficiency Disrupts Folate Metabolism: Insights from Antibody-Based Proteomic Analysis"
**作者**: Lee H, et al.
**摘要**: 文章利用针对MTHFSD N端的单克隆抗体，通过免疫组化和蛋白质印迹分析，揭示了MTHFSD缺乏症患者细胞中单碳代谢通路的异常。抗体特异性通过siRNA敲低实验验证，为疾病机制研究提供工具。
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3. **文献名称**: "Development of Monoclonal Antibodies Against MTHFSD for Functional Studies in Cancer Models"
**作者**: Zhang R, et al.
**摘要**: 本研究开发了靶向MTHFSD N端的单克隆抗体，用于探究其在肿瘤代谢重编程中的作用。抗体在结直肠癌组织中的高表达验证了MTHFSD的潜在致癌性，并证明其与患者预后相关。
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4. **文献名称**: "Epitope Mapping and Functional Analysis of MTHFSD-Specific Antibodies"
**作者**: Garcia M, et al.
**摘要**: 通过表位定位技术，确定了一种抗MTHFSD N端抗体的结合区域(氨基酸1-50)。该抗体被用于免疫沉淀实验，揭示了MTHFSD与甲硫氨酸合成酶(MTR)的相互作用，提示其在叶酸循环中的调控作用。
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**备注**：以上文献信息为示例性内容，实际研究中请通过PubMed或Google Scholar检索关键词“MTHFSD antibody”、“MTHFSD N-terminal”等获取准确文献。若研究较少，可扩展至相关代谢酶(如MTHFR、MTHFD1)的抗体验证文献作为参考。
The MTHFSD (N-term) antibody is a polyclonal or monoclonal antibody specifically designed to target the N-terminal region of the methylenetetrahydrofolate synthetase domain-containing (MTHFSD) protein. MTHFSD is an enzyme involved in one-carbon metabolism, a critical pathway for nucleotide synthesis, methylation reactions, and cellular redox homeostasis. The N-terminal domain of MTHFSD is essential for its enzymatic activity, facilitating the conversion of methenyltetrahydrofolate to methylenetetrahydrofolate, a key step in folate cycling.
This antibody is typically generated using immunogens derived from synthetic peptides or recombinant proteins corresponding to the N-terminal sequence of human MTHFSD. It is widely used in research applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect MTHFSD expression, localization, and regulation in various tissues and cell types. Its specificity for the N-terminal region ensures minimal cross-reactivity with other folate-related enzymes.
Studies employing the MTHFSD (N-term) antibody have contributed to understanding its role in cellular proliferation, mitochondrial function, and diseases like cancer or metabolic disorders. Validation via knockout controls or siRNA silencing is recommended to confirm signal specificity. The antibody serves as a vital tool for exploring MTHFSD's molecular mechanisms and potential therapeutic targets.
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