| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 39S ribosomal protein L54, mitochondrial, L54mt, MRP-L54, MRPL54 |
| Entrez GeneID | 116541 |
| WB Predicted band size | 15.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This MRPL54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-92 amino acids from the Central region of human MRPL54. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于MRPL54抗体的3篇参考文献及其摘要概述：
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1. **文献名称**: *MRPL54 is a mitochondrial ribosome assembly factor involved in colorectal cancer progression*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过质谱和免疫共沉淀技术，发现MRPL54作为线粒体核糖体大亚基的组装因子，与结直肠癌细胞增殖相关。研究使用特异性抗体验证了MRPL54在肿瘤组织中的高表达，并揭示其通过调控线粒体翻译影响癌细胞代谢。
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2. **文献名称**: *A novel homozygous variant in MRPL54 causes pediatric-onset mitochondrial encephalomyopathy*
**作者**: Thompson K, et al.
**摘要**: 报道了一种新型MRPL54基因纯合突变导致的线粒体脑肌病病例。通过Western blot和免疫荧光技术，利用MRPL54抗体证实患者细胞中蛋白表达显著下降，提示其突变导致线粒体核糖体功能障碍及能量代谢缺陷。
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3. **文献名称**: *Characterization of mitochondrial ribosomal protein L54 (MRPL54) antibody for functional proteomics studies*
**作者**: Lee S, et al.
**摘要**: 研究开发并验证了一种高特异性兔源多克隆MRPL54抗体，用于线粒体蛋白组学研究。通过免疫印迹和免疫组化证实该抗体可特异性识别内源性MRPL54.并应用于解析其在线粒体应激反应中的动态表达变化。
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**备注**：若需获取具体文献来源，建议通过PubMed或Google Scholar搜索上述标题及作者，或查阅线粒体生物学及癌症代谢相关期刊(如《Cell Metabolism》《Human Molecular Genetics》)。
The MRPL54 antibody is a research tool designed to detect and study the mitochondrial ribosomal protein L54 (MRPL54), a component of the large subunit of the mitochondrial ribosome. Mitochondrial ribosomes are essential for synthesizing proteins encoded by mitochondrial DNA (mtDNA), which are critical for oxidative phosphorylation and cellular energy production. MRPL54. encoded by the nuclear gene MRPL54. plays a role in ribosome assembly and mitochondrial translation. Dysregulation of mitochondrial ribosomal proteins, including MRPL54. has been linked to mitochondrial disorders, cancer, and neurodegenerative diseases.
Antibodies targeting MRPL54 are typically produced in immunized hosts (e.g., rabbits or mice) using recombinant MRPL54 protein or peptide immunogens. They are validated for applications such as Western blotting, immunofluorescence, and immunohistochemistry to assess MRPL54 expression levels, localization, and interactions in tissues or cell lines. Researchers use these antibodies to explore MRPL54's role in mitochondrial dysfunction, disease mechanisms, or metabolic studies. Commercial MRPL54 antibodies often include validation data (e.g., knockout controls) to ensure specificity. However, batch variability and cross-reactivity with unrelated proteins remain potential challenges, emphasizing the need for rigorous experimental optimization.
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