WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | TBC1 domain family member 10B, Rab27A-GAP-beta, TBC1D10B |
Entrez GeneID | 26000 |
WB Predicted band size | 87.2kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This TBC1D10B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 781-808 amino acids from the C-terminal region of human TBC1D10B. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TBC1D10B抗体的3篇参考文献摘要:
1. **文献名称**: *"TBC1D10B regulates EGFR degradation by interacting with the ESCRT-II complex"*
**作者**: Zhang Y, et al.
**摘要**: 本研究揭示了TBC1D10B通过结合ESCRT-II复合体调控表皮生长因子受体(EGFR)的内吞和溶酶体降解过程,文中使用特异性抗体验证了TBC1D10B在HeLa细胞中的定位及其与ESCRT-II的相互作用。
2. **文献名称**: *"A novel role of TBC1D10B in insulin-stimulated glucose uptake via GLUT4 translocation"*
**作者**: Lee S, et al.
**摘要**: 该研究通过免疫共沉淀和Western blot技术,利用TBC1D10B抗体证明其在脂肪细胞中调控胰岛素信号通路,促进GLUT4囊泡向细胞膜转运,从而增强葡萄糖摄取。
3. **文献名称**: *"TBC1D10B as a tumor suppressor: Antibody-based detection in colorectal cancer tissues"*
**作者**: Chen H, et al.
**摘要**: 通过免疫组化分析,发现TBC1D10B在结直肠癌组织中表达显著下调,其抗体检测结果与患者预后呈正相关,提示其可能通过抑制RAB35依赖的细胞迁移发挥抑癌作用。
(注:上述文献为模拟示例,实际引用需以具体数据库检索结果为准。)
**Background of TBC1D10B Antibody**
TBC1D10B (TBC1 domain family member 10B) is a protein encoded by the *TBC1D10B* gene, belonging to the TBC (Tre-2/Bub2/Cdc16) domain-containing family. Members of this family are characterized by their conserved TBC domain, which often functions as a GTPase-activating protein (GAP) for Rab GTPases, regulators of intracellular vesicular trafficking and membrane dynamics. TBC1D10B, also known as EPI64B or CARMIL2. has been implicated in cellular processes such as endocytosis, cell migration, and signal transduction. Studies suggest its role in actin cytoskeleton remodeling and immune synapse formation, particularly in T-cell activation and function.
Antibodies targeting TBC1D10B are essential tools for investigating its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study its involvement in physiological and pathological contexts. Research highlights TBC1D10B's potential links to cancer, immune disorders, and neurodegenerative diseases, where dysregulation of vesicular trafficking or cytoskeletal organization may contribute to disease progression. These antibodies aid in elucidating its mechanistic roles, including interactions with binding partners like clathrin or actin-regulatory proteins. Commercial TBC1D10B antibodies are typically validated for specificity and application compatibility, enabling researchers to explore its tissue distribution, post-translational modifications, and functional relevance in disease models.
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