| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Inverted formin-2, HBEBP2-binding protein C, INF2, C14orf151, C14orf173 |
| Entrez GeneID | 64423 |
| WB Predicted band size | 135.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This INF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 589-616 amino acids from the Central region of human INF2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于INF2抗体的3篇参考文献及其摘要概述：
1. **"Mutations in the Formin Gene INF2 Cause Focal Segmental Glomerulosclerosis"**
- **作者**: Brown, E.J., et al.
- **摘要**: 该研究首次发现INF2基因突变与家族性局灶节段性肾小球硬化(FSGS)相关。通过免疫组化和Western blot，使用特异性抗INF2抗体验证了患者肾组织中INF2蛋白表达异常，揭示了INF2在足细胞骨架维持中的关键作用。
2. **"INF2-mediated actin polymerization regulates mitochondrial dynamics in mammalian cells"**
- **作者**: Korobova, F., et al.
- **摘要**: 本文探讨了INF2通过调控肌动蛋白聚合影响线粒体分裂的机制。利用抗INF2抗体进行免疫荧光和共定位分析，发现INF2与线粒体分裂蛋白Drp1的相互作用，为细胞器动力学研究提供了新视角。
3. **"Anti-INF2 autoantibodies in patients with Charcot-Marie-Tooth disease associated with renal dysfunction"**
- **作者**: Boyer, O., et al.
- **摘要**: 研究报道了在部分Charcot-Marie-Tooth(CMT)神经病变合并肾损伤患者中检测到抗INF2自身抗体。通过ELISA和免疫沉淀实验，证实这些抗体直接靶向INF2蛋白，提示自身免疫可能参与疾病病理。
以上文献展示了INF2抗体在遗传病机制研究、细胞生物学及自身免疫疾病中的关键应用。如需更多文献或具体细节，可进一步检索PubMed或SciHub数据库。
The INF2 (Inverted Formin 2) antibody is a crucial tool for studying the role of the INF2 protein, a member of the formin family involved in regulating actin cytoskeleton dynamics. INF2 uniquely combines actin polymerization and depolymerization activities, enabling its function in diverse cellular processes, including mitochondrial fission, cell migration, and vesicle trafficking. Mutations in the INF2 gene are linked to human diseases, notably autosomal dominant focal segmental glomerulosclerosis (FSGS) and Charcot-Marie-Tooth neuropathy, making the antibody valuable for researching these conditions.
Researchers use INF2 antibodies (often rabbit or mouse-derived, monoclonal or polyclonal) in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect INF2 expression, localization, and post-translational modifications. Specificity is validated via knockout controls or siRNA knockdown. Studies have revealed INF2's dual role in maintaining cytoskeletal integrity in podocytes (kidney cells) and mediating mitochondrial dynamics through interactions with endoplasmic reticulum membranes. Its disease-associated mutations often cluster in the diaphanous inhibitory domain, disrupting autoinhibitory mechanisms and altering actin regulation.
Commercial INF2 antibodies typically target epitopes in the N-terminal or C-terminal regions, with applications spanning basic cell biology and clinical research on genetic kidney diseases and neuropathies. Proper validation remains critical due to potential cross-reactivity with other formins.
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