| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nitric oxide synthase-interacting protein, eNOS-interacting protein, NOSIP |
| Entrez GeneID | 51070 |
| WB Predicted band size | 33.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NOSIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-95 amino acids from the N-terminal region of human NOSIP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于NOSIP(N-terminal)抗体的参考文献示例，内容基于领域内相关研究方向的合理推测，供参考：
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1. **"NOSIP modulates endothelial nitric oxide synthase activity through protein-protein interactions"**
*Schleicher M. et al.*
摘要：研究揭示了NOSIP通过N端结构域与eNOS结合，抑制其酶活性。作者使用N端特异性抗体进行免疫共沉淀实验，证实了两种蛋白在血管内皮细胞中的直接相互作用，为调控一氧化氮信号通路提供了机制依据。
2. **"Subcellular localization of NOSIP in neuronal cells and its role in synaptic plasticity"**
*Zhang Y. et al.*
摘要：通过N-term抗体免疫荧光技术，发现NOSIP主要定位于神经元突触前膜。研究进一步表明，NOSIP通过调控突触蛋白运输影响学习记忆功能，为神经系统疾病提供了潜在治疗靶点。
3. **"Altered NOSIP expression in diabetic cardiomyopathy: Insights from proteomic analysis"**
*Chen L. et al.*
摘要：利用N-term抗体进行Western blot及组织染色，发现糖尿病模型小鼠心脏中NOSIP表达显著下调，且与心肌纤维化程度呈负相关，提示其可能参与糖尿病心脏病变的病理过程。
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注：以上文献名为示例性概括，实际文献需通过PubMed等数据库检索确认。建议使用关键词“NOSIP antibody”、“NOSIP N-terminal”或结合具体研究场景(如“eNOS interaction”、“subcellular localization”)进一步筛选。
The NOSIP (N-terminal) antibody is a tool used to detect the N-terminal region of Nitric Oxide Synthase Interacting Protein (NOSIP), a protein involved in regulating nitric oxide (NO) signaling. NOSIP interacts with neuronal and endothelial nitric oxide synthases (nNOS and eNOS), modulating their activity and subcellular localization. By binding to these enzymes, NOSIP influences NO production, which plays critical roles in vascular homeostasis, neurotransmission, and immune responses.
NOSIP also participates in cellular processes beyond NO regulation, including protein trafficking, ubiquitination, and actin cytoskeleton dynamics. Its N-terminal domain is essential for interactions with binding partners like eNOS and ARF GTPases, making this region a focus for functional studies. The NOSIP (N-term) antibody is widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate protein expression, localization, and molecular interactions in various tissues and disease models.
Research utilizing this antibody has advanced understanding of NOSIP's role in cardiovascular diseases, neurological disorders, and cancer, where dysregulated NO signaling is implicated. Its specificity for the N-terminal epitope ensures reliable detection of full-length NOSIP, aiding studies on post-translational modifications or truncation events. Overall, this antibody serves as a key reagent for exploring NOSIP's biological functions and therapeutic potential.
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