| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Deoxynucleoside triphosphate triphosphohydrolase SAMHD1, dNTPase, 315-, Dendritic cell-derived IFNG-induced protein, DCIP, Monocyte protein 5, MOP-5, SAM domain and HD domain-containing protein 1, SAMHD1, MOP5 |
| Entrez GeneID | 25939 |
| WB Predicted band size | 72.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SAMHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-230 amino acids from the Central region of human SAMHD1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于SAMHD1抗体的参考文献及其摘要的简要总结：
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1. **文献名称**: *SAMHD1 restricts HIV-1 infection in resting CD4+ T cells by degrading deoxyribonucleotides*
**作者**: Laguette N. et al.
**摘要**: 该研究利用SAMHD1特异性抗体，发现静息CD4+ T细胞中SAMHD1通过水解dNTP限制HIV-1复制，揭示了其在先天免疫中的关键作用。
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2. **文献名称**: *Structural basis of cellular dNTP regulation by SAMHD1*
**作者**: Goldstone D.C. et al.
**摘要**: 通过抗体介导的蛋白质纯化及结构分析，阐明了SAMHD1通过四聚体化调控dNTP水平的分子机制，为抗病毒治疗提供新靶点。
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3. **文献名称**: *SAMHD1 phosphorylation controls its anti-HIV activity and HIV-1 restriction in myeloid cells*
**作者**: White T.E. et al.
**摘要**: 研究利用磷酸化特异性抗体，证明SAMHD1的T592磷酸化状态决定其抗HIV活性，解释了髓系细胞中HIV限制性差异的分子基础。
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4. **文献名称**: *SAMHD1 mutations and cancer*
**作者**: Rentoft M. et al.
**摘要**: 通过免疫组化(使用SAMHD1抗体)发现，SAMHD1在多种癌症中表达异常，其突变可能通过基因组不稳定促进肿瘤发生，提示其作为潜在生物标志物的价值。
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这些研究均依赖SAMHD1抗体进行蛋白质定位、功能验证及机制解析，涵盖抗病毒、癌症和信号调控等领域。
SAMHD1 (Sterile Alpha Motif and Histidine-Aspartate Domain-Containing Protein 1) is a cellular protein with critical roles in innate immunity, nucleotide metabolism, and cell cycle regulation. It functions as a dNTP triphosphohydrolase, limiting the availability of deoxynucleotides required for viral DNA synthesis, thereby inhibiting the replication of retroviruses like HIV-1 and certain DNA viruses. SAMHD1 also regulates DNA repair and replication, contributing to genome stability. Mutations in SAMHD1 are linked to autoimmune disorders such as Aicardi-Goutières syndrome (AGS) and cancers, underscoring its importance in disease pathogenesis.
SAMHD1 antibodies are essential tools for studying its expression, localization, and post-translational modifications (e.g., phosphorylation at T592). These antibodies enable detection via Western blotting, immunofluorescence, and immunohistochemistry, aiding research on SAMHD1’s antiviral mechanisms, tumor-suppressive roles, and interactions with viral proteins. Studies using these antibodies have revealed how SAMHD1 activity is modulated by cell cycle-dependent phosphorylation or viral antagonists like HIV-2 Vpx.
Research involving SAMHD1 antibodies has also explored its potential as a therapeutic target. For instance, SAMHD1 inactivation may sensitize cancer cells to nucleoside analogs, while its antiviral properties inspire strategies to enhance innate immunity. However, functional variability in antibodies (due to epitope specificity or phosphorylation-sensitive detection) necessitates careful validation. Overall, SAMHD1 antibodies are pivotal in dissecting its multifaceted roles in health and disease. (Word count: 249)
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