| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein IMPACT, Imprinted and ancient gene protein homolog, IMPACT |
| Entrez GeneID | 55364 |
| WB Predicted band size | 36.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IMPACT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human IMPACT. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与 **IMPACT (N-term)抗体** 相关的参考文献示例(注：文献为示例性概括，非真实存在)：
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1. **文献名称**: *"Characterization of IMPACT protein in neuronal development using a novel N-terminal specific antibody"*
**作者**: Rodrigues et al. (2005)
**摘要**: 开发了一种针对IMPACT蛋白N末端的高特异性多克隆抗体，并通过Western blot和免疫组化验证其在啮齿类动物脑组织中的表达模式，发现IMPACT在胚胎神经发育中显著上调。
2. **文献名称**: *"IMPACT interacts with axonal guidance molecules: Insights from N-terminal epitope tagging studies"*
**作者**: Tcherkezian et al. (2012)
**摘要**: 利用N端特异性抗体研究IMPACT与Netrin-1信号通路的相互作用，证明其在轴突导向中的功能，并通过免疫共沉淀(Co-IP)验证抗体对天然蛋白的识别能力。
3. **文献名称**: *"Antibody validation for IMPACT subcellular localization: A focus on N-terminal isoforms"*
**作者**: Smith et al. (2018)
**摘要**: 系统验证了商用IMPACT N端抗体(货号：Abcam ab123XX)在免疫荧光(IF)和免疫印迹中的特异性，发现其可区分不同剪切变体，并适用于人源细胞系模型。
4. **文献名称**: *"IMPACT in neurodegenerative disorders: Role of domain-specific antibody tools"*
**作者**: Lee & Kim (2020)
**摘要**: 综述了IMPACT蛋白在阿尔茨海默病中的潜在作用，强调N端抗体在病理组织检测中的应用，并比较了不同物种间抗体交叉反应性的差异。
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如需真实文献，建议通过 **PubMed** 或 **Google Scholar** 检索关键词：**"IMPACT protein antibody N-terminal"** 或 **"anti-IMPACT (N-term) validation"**。
IMPACT (N-term) antibodies are immunological tools designed to target the N-terminal region of the IMPACT protein, a conserved eukaryotic protein implicated in regulating cellular processes such as translation, neuronal development, and stress responses. IMPACT (Imprinted and Ancient) is highly expressed in neurons and germ cells, where it modulates protein synthesis by interacting with the GCN2 kinase pathway, influencing amino acid starvation responses and mTOR signaling. Its N-terminal domain is critical for binding to specific partners, including GCN1. which facilitates its regulatory role in translation.
These antibodies are commonly used in research to detect endogenous IMPACT expression via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Their specificity for the N-terminal region ensures recognition of full-length IMPACT, avoiding cross-reactivity with truncated isoforms or degradation products. Validated in multiple species (e.g., human, mouse, rat), IMPACT (N-term) antibodies help elucidate the protein’s function in neurodevelopment, metabolic adaptation, and diseases like cancer or neurodegenerative disorders. Researchers rely on these antibodies to study IMPACT’s involvement in cellular stress resilience, stem cell maintenance, and epigenetic regulation, leveraging their ability to provide spatial and quantitative protein expression data in diverse experimental models.
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