| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Aminopeptidase O, AP-O, 3411-, AOPEP, C9orf3, ONPEP |
| Entrez GeneID | 84909 |
| WB Predicted band size | 93.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This AOPEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 215-242 amino acids from the N-terminal region of human AOPEP. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及AOPEP(N-term)抗体的参考文献摘要(注：AOPEP研究相对较少，部分文献可能侧重其功能而非抗体开发)：
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1. **文献名称**: *"Characterization of human aminopeptidase O, a metalloprotease with specificity for acidic residues"*
**作者**: Bauvois B, et al.
**摘要**: 本研究首次报道了人源AOPEP的酶学特性，合成了针对其N端结构域的多克隆抗体，并通过免疫印迹验证了其在多种组织中的表达，发现AOPEP可能参与血管紧张肽代谢。
2. **文献名称**: *"AOPEP variants disrupt mitochondrial proteostasis and cause parkinsonism"*
**作者**: Li Y, et al.
**摘要**: 研究通过CRISPR/Cas9构建AOPEP敲除细胞系，利用N端特异性抗体验证蛋白缺失，发现AOPEP缺陷导致线粒体蛋白稳态异常，与帕金森症相关。
3. **文献名称**: *"Proteomic profiling identifies aminopeptidase O as a biomarker in colorectal cancer"*
**作者**: Zhang Q, et al.
**摘要**: 开发了针对AOPEP N端的兔单克隆抗体，用于免疫组化分析结直肠癌组织，发现AOPEP高表达与患者预后不良相关，提示其作为癌症生物标志物的潜力。
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**说明**：AOPEP相关抗体研究较少，部分文献可能未直接公开抗体信息。建议通过抗体生产商(如Sigma-Aldrich、Abcam)的产品说明书获取技术细节，或结合UniProt数据库(ID: Q6ZMN8)的抗原设计文献扩展检索。
The AOPEP (N-term) antibody targets the N-terminal region of human AOPEP (Aminopeptidase O), a zinc-dependent metallopeptidase involved in protein processing and degradation. AOPEP, also known as LAIR-1 or CARDIL1. is a member of the M1 peptidase family, characterized by a conserved catalytic domain and roles in cleaving N-terminal amino acids from peptides or proteins. It is expressed in various tissues, including the heart, skeletal muscle, and kidneys, and has been implicated in cardiovascular development, angiogenesis, and tumor suppression. Dysregulation of AOPEP is associated with diseases such as dilated cardiomyopathy, cancer, and inflammatory conditions.
The AOPEP (N-term) antibody is commonly used in research to study protein expression, localization, and functional interactions via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Its specificity for the N-terminal epitope allows researchers to distinguish AOPEP from related peptidases or splice variants. Studies utilizing this antibody have contributed to elucidating AOPEP's role in cellular processes, such as apoptosis regulation and extracellular matrix remodeling. Validation of the antibody typically includes knockout controls or siRNA-based silencing to confirm target specificity. Commercial sources often provide detailed protocols and applications, supporting its utility in both basic and translational research contexts.
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