WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | 45 kDa calcium-binding protein, Cab45, Stromal cell-derived factor 4, SDF-4, SDF4, CAB45 |
Entrez GeneID | 51150 |
WB Predicted band size | 41.8kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This SDF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-287 amino acids from the C-terminal region of human SDF4. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SDF4抗体的虚构参考文献示例,格式符合要求,内容基于假设性研究:
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1. **文献名称**:*SDF4 Antibody Development and Application in Calcium Signaling Studies*
**作者**:Zhang L, et al.
**摘要**:本研究报道了一种新型SDF4单克隆抗体的开发,验证了其在Western blot和免疫荧光中的特异性。实验表明,SDF4在HeLa细胞的钙离子调控中高表达,抗体成功用于检测其在内质网定位的动态变化。
2. **文献名称**:*SDF4 as a Biomarker in Colorectal Cancer: Immunohistochemical Analysis*
**作者**:Smith J, et al.
**摘要**:通过商业化SDF4抗体对120例结直肠癌组织进行免疫组化分析,发现SDF4高表达与患者生存率负相关,提示其可能作为潜在预后标志物。
3. **文献名称**:*Role of SDF4 in Secretory Pathway Dysregulation: Insights from Knockout Models*
**作者**:Tanaka K, et al.
**摘要**:利用SDF4特异性抗体研究基因敲除细胞系,发现SDF4缺失导致高尔基体钙稳态紊乱,影响蛋白质分泌功能,证实其在分泌途径中的关键作用。
4. **文献名称**:*SDF4 Antibody-Based ELISA for Early Diagnosis of Cardiovascular Disease*
**作者**:Wang Y, et al.
**摘要**:开发了一种基于SDF4多克隆抗体的ELISA检测法,临床样本分析显示血清SDF4水平与动脉粥样硬化严重程度显著相关,为无创诊断提供新方法。
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注:上述文献为示例性内容,实际研究中请通过学术数据库(如PubMed)检索真实文献。若需进一步协助验证,请提供更具体的信息。
The SDF4 antibody targets Stromal Cell-Derived Factor 4 (SDF4), also known as calumenin, a member of the CREC (Cab45. reticulocalbin, ERC45. calumenin) family of calcium-binding proteins. SDF4 is characterized by six conserved EF-hand motifs, though only a subset binds calcium. It localizes to the endoplasmic reticulum (ER) and Golgi apparatus, where it regulates calcium homeostasis, protein folding, and secretory pathway functions. SDF4 interacts with enzymes like vitamin K epoxide reductase (VKOR), influencing γ-carboxylation processes critical for blood coagulation and bone metabolism.
Research highlights SDF4's involvement in cardiovascular diseases, cancer, and metabolic disorders. In cancer, SDF4 may promote tumor progression by modulating angiogenesis or metastasis-related pathways. Its dysregulation is also linked to platelet dysfunction and vascular calcification. Antibodies against SDF4 are vital tools for detecting its expression and localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These studies help elucidate SDF4's role in cellular physiology and pathology, with potential applications in biomarker discovery or therapeutic targeting. Despite its established roles, further research is needed to fully unravel its molecular mechanisms and tissue-specific functions.
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