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Rabbit Polyclonal HECTD3 Antibody

  • 中文名: HECTD3抗体
  • 别    名: E3 ubiquitin-protein ligase HECTD3, 632-, HECT domain-containing protein 3, HECTD3
货号: IPDX31807
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesE3 ubiquitin-protein ligase HECTD3, 632-, HECT domain-containing protein 3, HECTD3
Entrez GeneID79654
WB Predicted band size97.1kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis HECTD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 740-769 amino acids from the C-terminal region of human HECTD3.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于HECTD3抗体的3篇参考文献示例(内容为模拟概括,具体文献需根据实际检索补充):

1. **文献名称**:*HECTD3 regulates the inflammatory response through ubiquitination of TAB2*

**作者**:Li Y, et al.

**摘要**:该研究利用HECTD3特异性抗体,通过免疫沉淀和Western blot技术,揭示了HECTD3通过介导TAB2蛋白的泛素化修饰调控NF-κB信号通路,从而影响巨噬细胞的炎症反应。

2. **文献名称**:*HECTD3 promotes breast cancer metastasis by stabilizing Snail1*

**作者**:Wang X, et al.

**摘要**:研究通过免疫组化(使用HECTD3抗体)发现HECTD3在乳腺癌组织中高表达,并通过泛素化降解调控Snail1蛋白稳定性,促进上皮-间质转化(EMT)和肿瘤转移。

3. **文献名称**:*HECTD3 modulates antiviral immunity by targeting MAVS for degradation*

**作者**:Chen Z, et al.

**摘要**:该研究利用HECTD3敲除细胞系及抗体检测,证明HECTD3通过泛素化线粒体抗病毒信号蛋白(MAVS),抑制I型干扰素产生,影响宿主对RNA病毒的抗病毒反应。

4. **文献名称**:*The E3 ligase HECTD3 restricts neuroinflammation by promoting degradation of NLRP3*

**作者**:Zhang R, et al.

**摘要**:通过免疫共沉淀(Co-IP)结合HECTD3抗体,研究发现HECTD3能够泛素化降解NLRP3炎症小体,缓解小胶质细胞过度活化,为神经退行性疾病提供潜在治疗靶点。

**注意**:以上为模拟内容,实际文献需通过PubMed、Google Scholar等平台以关键词“HECTD3 antibody”或“HECTD3 ubiquitin ligase”检索,并筛选涉及该抗体应用的实验研究。

背景信息

The HECTD3 antibody is a crucial tool in studying the biological functions of HECTD3. a member of the HECT (Homologous to E6-AP C-Terminus) family of E3 ubiquitin ligases. HECTD3 plays a pivotal role in the ubiquitin-proteasome system, mediating substrate-specific protein ubiquitination, which regulates diverse cellular processes, including protein degradation, signal transduction, and immune responses. Structurally, HECTD3 contains an N-terminal domain involved in substrate recognition and a C-terminal HECT domain responsible for catalyzing ubiquitin transfer. Research has linked HECTD3 to critical pathways such as NF-κB activation, apoptosis, and DNA damage repair, with implications in cancer, inflammation, and neurodegenerative diseases.

The development of HECTD3-specific antibodies has enabled researchers to investigate its expression patterns, subcellular localization, and interaction partners. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to quantify HECTD3 levels in various tissues or disease models. Notably, dysregulation of HECTD3 has been observed in multiple cancers, where it may act as either an oncogene or tumor suppressor depending on cellular context. For instance, elevated HECTD3 expression correlates with poor prognosis in certain malignancies, while its downregulation is associated with impaired DNA repair mechanisms.

Validating HECTD3 antibody specificity remains essential due to structural similarities among HECT family members. Knockout/knockdown controls are often employed to confirm target specificity in experimental settings. Ongoing studies using these antibodies continue to unravel HECTD3's role in disease pathogenesis and its potential as a therapeutic target or diagnostic biomarker.

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