| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AT-rich interactive domain-containing protein 5B, ARID domain-containing protein 5B, MRF1-like protein, Modulator recognition factor 2, MRF-2, ARID5B, DESRT, MRF2 |
| Entrez GeneID | 84159 |
| WB Predicted band size | 132.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ARID5B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-418 amino acids from the Central region of human ARID5B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ARID5B抗体的3篇文献示例(内容基于模拟生成，非真实文献)：
1. **文献名称**：*ARID5B modulates inflammatory responses in macrophages via transcriptional regulation of NF-κB pathway*
**作者**：Li, X. et al.
**摘要**：研究利用ARID5B特异性抗体进行ChIP-seq和免疫沉淀实验，发现ARID5B通过结合特定炎症基因启动子区域调控巨噬细胞中NF-κB信号通路，揭示其在自身免疫疾病中的潜在作用。
2. **文献名称**：*ARID5B expression correlates with chemoresistance in pediatric acute lymphoblastic leukemia*
**作者**：Smith, J.R. et al.
**摘要**：通过ARID5B抗体进行免疫组化分析，发现ARID5B在儿童ALL患者中高表达，并与甲氨蝶呤耐药性显著相关，提示其作为治疗靶点的可能性。
3. **文献名称**：*Structural and functional characterization of ARID5B antibody for chromatin remodeling studies*
**作者**：Wang, Y. et al.
**摘要**：该研究验证了一种高特异性ARID5B单克隆抗体的性能，证明其在Western blot、免疫荧光和染色质结合实验中的可靠性，为后续机制研究提供工具支持。
4. **文献名称**：*ARID5B genetic variants and systemic lupus erythematosus susceptibility: A population-based study*
**作者**：Garcia, M. et al.
**摘要**：结合ARID5B抗体检测患者组织样本，发现特定SNP位点与ARID5B蛋白表达水平变化相关，进而影响系统性红斑狼疮的易感性。
(注：以上文献为示例，实际引用请通过学术数据库检索验证。)
The ARID5B (AT-rich interaction domain 5B) antibody is a research tool designed to detect and study the ARID5B protein, a member of the ARID family of DNA-binding proteins involved in transcriptional regulation and chromatin remodeling. ARID5B plays critical roles in embryonic development, cell differentiation, and immune response modulation. It binds to AT-rich promoter regions of target genes, influencing pathways related to adipogenesis, hematopoiesis, and inflammatory signaling. Dysregulation of ARID5B has been implicated in cancers (e.g., leukemia, neuroblastoma), autoimmune diseases (e.g., rheumatoid arthritis), and metabolic disorders.
ARID5B antibodies are typically produced in hosts like rabbits or mice using immunogenic peptide sequences derived from conserved regions of the human ARID5B protein (molecular weight ~120 kDa). These antibodies are validated for applications such as Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Specific clones (e.g., EPR17429. D6L8G) may target distinct epitopes (e.g., N-terminal or C-terminal domains). Researchers use these antibodies to explore ARID5B's role in gene regulation, its interaction with transcription factors like STAT3. and its association with disease mechanisms. Validation often includes knockout cell line controls to confirm specificity. Recent studies highlight its therapeutic potential in targeting ARID5B-overexpressing cancers or inflammatory conditions.
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