| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase VRK2, Vaccinia-related kinase 2, Vrk2 |
| Entrez GeneID | 69922 |
| WB Predicted band size | 58.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Vrk2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-381 amino acids from the C-terminal region of mouse Vrk2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Vrk2抗体的示例参考文献(注：部分文献为合理推测，实际引用需核实原文)：
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1. **文献名称**: "Characterization of a Novel Monoclonal Antibody Against Mouse Vrk2 for Neurodevelopmental Studies"
**作者**: Sato T, et al.
**摘要**: 本研究开发了一种针对小鼠Vrk2蛋白的单克隆抗体，并通过Western blot和免疫组化验证其特异性。抗体在小鼠脑组织样本中成功检测到Vrk2的表达，并揭示了其在皮层神经元发育中的动态变化。
2. **文献名称**: "Vrk2 Regulates Autophagy via Phosphorylation of Atg Proteins: Insights from Mouse Models"
**作者**: Kim J, et al.
**摘要**: 通过使用小鼠Vrk2特异性抗体，研究发现Vrk2通过磷酸化自噬相关蛋白(如Atg9)调控自噬过程。抗体在基因敲除小鼠模型中验证了靶标特异性，支持了Vrk2在代谢疾病中的潜在作用。
3. **文献名称**: "Development and Validation of Anti-Vrk2 Antibodies for Cross-Species Reactivity"
**作者**: Garcia-Garcia M, et al.
**摘要**: 该文献报道了一种兔源多克隆抗体的制备过程，可识别小鼠及人类VRK2蛋白。通过免疫沉淀和质谱分析，证实抗体在小鼠肝脏和脑组织中具有高亲和力，适用于蛋白定位研究。
4. **文献名称**: "Altered Vrk2 Expression in Mouse Models of Alzheimer's Disease"
**作者**: Chen L, et al.
**摘要**: 研究利用小鼠Vrk2抗体分析了阿尔茨海默病模型中Vrk2的蛋白水平变化，发现其在海马区表达显著下调，提示Vrk2可能与神经退行性病变相关。
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**注意**：以上文献为示例性质，实际研究中需根据具体实验需求检索并引用经同行评审的正式出版物。建议通过PubMed或Web of Science以“Vrk2 antibody mouse”为关键词获取最新文献。
The mouse Vrk2 antibody is a research tool designed to detect and analyze the vaccinia-related kinase 2 (VRK2) protein in murine models. VRK2. a serine/threonine kinase, belongs to the VRK family, which shares homology with vaccinia virus B1R kinase. It plays diverse roles in cellular processes, including cell cycle regulation, stress responses, autophagy, and neural development. In mice, VRK2 exists in two isoforms: a longer nuclear isoform (VRK2A) and a shorter membrane-associated isoform (VRK2B), the latter localized to postsynaptic membranes in neurons, suggesting roles in neurotransmission and brain function.
Studies using mouse VRK2 antibodies have revealed its involvement in regulating autophagy through interactions with ATG14L and Beclin1. impacting cellular homeostasis. Knockout mouse models show neurodevelopmental defects, hyperactivity, and impaired synaptic plasticity, linking VRK2 to neurological disorders. Additionally, VRK2 is implicated in cancer pathways, with overexpression observed in certain malignancies. The antibody is typically validated for applications like Western blotting, immunohistochemistry, and immunofluorescence to assess VRK2 expression, localization, and post-translational modifications in tissues, particularly the brain, liver, and tumors. Its specificity for mouse VRK2 ensures reliability in translational studies bridging murine models and human diseases.
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