| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Liprin-alpha-1, LAR-interacting protein 1, LIP-1, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-1, PTPRF-interacting protein alpha-1, PPFIA1, LIP1 |
| Entrez GeneID | 8500 |
| WB Predicted band size | 135.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PPFIA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-80 amino acids from the N-terminal region of human PPFIA1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PPFIA1 (N-term)抗体的3篇参考文献示例(内容为模拟虚构，供参考)：
1. **文献名称**: "PPFIA1 N-terminal antibody validation in synaptic protein complexes"
**作者**: Smith A, et al.
**摘要**: 本研究验证了针对PPFIA1 N端结构域抗体的特异性，通过免疫沉淀和质谱分析揭示了PPFIA1与LAR-RPTPs在神经元突触中的相互作用，并证明其在调控突触可塑性中的功能。
2. **文献名称**: "Role of PPFIA1 in cancer metastasis studied via N-terminal targeted antibody"
**作者**: Chen L, et al.
**摘要**: 利用PPFIA1 N端抗体进行免疫组化和Western blot分析，发现PPFIA1在乳腺癌细胞中高表达，并通过调控细胞黏附通路促进转移，抗体特异性通过siRNA敲低实验证实。
3. **文献名称**: "Developmental expression profiling of PPFIA1 using N-term-specific antibodies"
**作者**: Tanaka K, et al.
**摘要**: 通过N端特异性抗体追踪PPFIA1在小鼠胚胎发育中的时空表达模式，发现其在神经嵴细胞迁移和皮质神经元分化中起关键作用，抗体特异性经敲除小鼠模型验证。
4. **文献名称**: "PPFIA1 antibody cross-reactivity analysis across species"
**作者**: Müller R, et al.
**摘要**: 评估了PPFIA1 N端抗体在人类、小鼠和大鼠样本中的交叉反应性，确认其适用于多物种研究，并应用于阿尔茨海默病模型中淀粉样蛋白沉积相关通路分析。
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注：以上文献信息为模拟生成，实际引用需查询真实数据库(如PubMed)。建议通过关键词“PPFIA1 antibody N-terminal”或“PPFIA1 epitope mapping”检索最新研究。
The PPFIA1 (N-term) antibody specifically targets the N-terminal region of protein tyrosine phosphatase receptor type F polypeptide-interacting protein alpha-1 (PPFIA1), a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. PPFIA1 plays critical roles in regulating cell adhesion, synaptic organization, and intracellular signaling by interacting with LAR-family phosphatases and other partners like β-catenin or GRIP1. It is implicated in diverse biological processes, including neuronal development, cancer metastasis, and immune response modulation.
Antibodies against the N-terminal domain of PPFIA1 are commonly used to study its expression, localization, and interactions in various experimental models (e.g., Western blot, immunofluorescence, immunoprecipitation). The N-terminal region is often chosen to avoid cross-reactivity with other liprin family members and to detect specific isoforms. Research involving this antibody has shed light on PPFIA1's overexpression in certain cancers (e.g., breast, lung) and its role in synaptic plasticity. Commercial antibodies are typically validated using knockout controls or tagged proteins to ensure specificity. For precise applications, researchers should refer to datasheets detailing host species, clonality, and validation data from public databases like UniProt or the Human Protein Atlas.
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