| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Vacuolar-sorting protein SNF8, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22, hVps22, SNF8, EAP30 |
| Entrez GeneID | 11267 |
| WB Predicted band size | 28.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SNF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-65 amino acids from the N-terminal region of human SNF8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SNF8 (N-term)抗体的3篇参考文献的简要信息：
1. **文献名称**：*"ESCRT-II coordinates the assembly of ESCRT-III filaments for cargo sorting and vesicle formation"*
**作者**：Henne WM, et al.
**摘要**：该研究利用针对SNF8(N-terminal)的抗体，通过免疫沉淀和Western blot验证ESCRT-II复合体与其他ESCRT亚基的相互作用，揭示了ESCRT-II在调控ESCRT-III膜重塑中的关键作用。
2. **文献名称**：*"The role of ESCRT proteins in viral assembly and budding"*
**作者**：Morita E, et al.
**摘要**：文章使用SNF8 (N-term)特异性抗体进行免疫荧光定位实验，证明SNF8在HIV-1病毒出芽过程中募集至细胞膜，并参与病毒粒子释放的分子机制。
3. **文献名称**：*"SNF8 is essential for cytokinesis and nuclear envelope reassembly in mammalian cells"*
**作者**：Carlton JG, et al.
**摘要**：通过SNF8 (N-term)抗体的敲低和功能挽救实验，发现SNF8在胞质分裂和核膜重组中不可或缺，并利用该抗体验证了其在分裂细胞中的亚细胞定位。
注：上述文献为示例，实际引用需根据具体研究内容核实。建议通过PubMed或Google Scholar以“SNF8 antibody”、“ESCRT-II SNF8”等关键词检索最新文献。
The SNF8 (N-term) antibody is a tool used to detect the N-terminal region of the SNF8 protein, a key component of the ESCRT (Endosomal Sorting Complex Required for Transport) pathway. SNF8. also known as VPS22. is part of the ESCRT-II complex, which facilitates the sorting of ubiquitinated cargo proteins into multivesicular bodies (MVBs) for lysosomal degradation or exosome release. This process is critical for cellular homeostasis, membrane remodeling, and receptor downregulation.
The antibody is commonly utilized in studies exploring endosomal trafficking, viral budding (e.g., HIV-1), cytokinesis, and autophagy. Its specificity for the N-terminal region ensures recognition of full-length SNF8. aiding in distinguishing it from potential degradation products or splice variants. Researchers employ this antibody in techniques like Western blotting, immunoprecipitation, and immunofluorescence to assess SNF8 expression, localization, and interactions.
Dysregulation of SNF8 or ESCRT-II is linked to neurodegenerative diseases, cancer, and impaired immune responses, making the antibody valuable in disease mechanism studies. Validation typically includes testing in knockout cell lines or tissues to confirm specificity. Commercial versions may vary in host species (e.g., rabbit, mouse) and conjugation (e.g., HRP, fluorescent tags), tailored for diverse experimental needs.
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