| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33, GPR84, EX33 |
| Entrez GeneID | 53831 |
| WB Predicted band size | 43.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GPR84 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-293 amino acids from the Central region of human GPR84. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于GPR84抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *GPR84 is a critical receptor for lysophosphatidic acid-mediated calcium signaling in human monocytes*
**作者**: Akira Takeda, et al.
**摘要**: 本研究验证了一种高特异性GPR84抗体的应用，证实GPR84在单核细胞中通过溶血磷脂酸激活钙信号通路。抗体通过免疫印迹和流式细胞术验证，揭示了GPR84在炎症反应中的调控作用。
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2. **文献名称**: *Development and characterization of a selective GPR84 antagonist antibody for metabolic disease research*
**作者**: MilliporeSigma Antibody Validation Team
**摘要**: 文章报道了一种新型GPR84拮抗性抗体的开发，该抗体通过阻断中链脂肪酸(如癸酸)与受体的结合，抑制巨噬细胞炎症反应。抗体经ELISA和免疫荧光验证，用于肥胖相关代谢紊乱研究。
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3. **文献名称**: *GPR84 promotes pulmonary fibrosis by driving profibrotic macrophage activation*
**作者**: Yukiko Suzuki, et al.
**摘要**: 研究利用抗GPR84抗体进行组织免疫染色，发现GPR84在肺纤维化患者的巨噬细胞中高表达，并证实其通过激活TGF-β信号通路促进纤维化进程。抗体特异性经siRNA敲除实验验证。
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(注：以上文献信息为模拟示例，实际文献需通过PubMed或Google Scholar检索确认。)
GPR84 is a member of the G protein-coupled receptor (GPCR) family, primarily expressed in immune cells such as macrophages, neutrophils, and dendritic cells. It plays a role in inflammatory responses and metabolic regulation, activated by endogenous medium-chain fatty acids (e.g., decanoic acid, lauric acid) or synthetic agonists. GPR84 signaling is linked to pro-inflammatory pathways, potentially influencing immune cell migration, cytokine production, and metabolic diseases like obesity and insulin resistance.
GPR84 antibodies are essential tools for studying its expression, localization, and function. They enable detection of the receptor in tissues or cells via techniques like Western blotting, immunohistochemistry, or flow cytometry. These antibodies help explore GPR84's role in disease models, such as chronic inflammation, fibrosis, and cancer, where its dysregulation may contribute to pathogenesis.
Recent research highlights GPR84 as a potential therapeutic target. Antibodies targeting specific epitopes can modulate receptor activity, aiding in mechanistic studies or drug discovery. However, challenges remain in understanding its dual pro- and anti-inflammatory roles, ligand-binding specificity, and tissue-specific effects. High-quality, validated GPR84 antibodies are critical for clarifying these complexities and advancing translational applications in immune-metabolic disorders.
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