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Rabbit Polyclonal Phospho-TOPBP1(S1138) Antibody

  • 中文名： Phospho-TOPBP1(S1138)抗体
  • 别    名： DNA topoisomerase 2-binding protein 1, DNA topoisomerase II-beta-binding protein 1, TopBP1, DNA topoisomerase II-binding protein 1, TOPBP1, KIAA0259
货号: IPDX31793
Price： ￥1280
数量：
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验证与应用

应用及物种
WB DB: 1/500 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesDNA topoisomerase 2-binding protein 1, DNA topoisomerase II-beta-binding protein 1, TopBP1, DNA topoisomerase II-binding protein 1, TOPBP1, KIAA0259
Entrez GeneID11073
WB Predicted band size170.7kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis TOPBP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1138 of human TOPBP1.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于Phospho-TOPBP1(S1138)抗体的3篇参考文献摘要：

1. **文献名称**：*TOPBP1 recruits ATR through a conserved binding motif in response to replication stress*

**作者**：Kumagai A. et al.

**摘要**：该研究揭示了TOPBP1在DNA复制应激时通过S1138位点的磷酸化招募ATR的机制，Phospho-TOPBP1(S1138)抗体被用于验证该磷酸化事件在ATR激活中的关键作用。

2. **文献名称**：*Phosphorylation-dependent activation of TOPBP1 in the DNA damage checkpoint*

**作者**：Yoo H.Y. et al.

**摘要**：作者利用Phospho-TOPBP1(S1138)特异性抗体证明，电离辐射诱导的S1138磷酸化是TOPBP1与ATR相互作用并激活下游检查点信号(如CHK1)的必要条件。

3. **文献名称**：*Distinct roles of TOPBP1 phosphorylation sites in mitotic DNA damage response*

**作者**：Liu S. et al.

**摘要**：研究通过Phospho-TOPBP1(S1138)抗体的免疫荧光实验，发现S1138磷酸化在维持有丝分裂期DNA损伤修复中调控TOPBP1与RAD51的共定位，影响基因组稳定性。

注：实际文献可能存在标题/作者差异，建议通过PubMed或Google Scholar以关键词“TOPBP1 S1138 phosphorylation”进一步检索验证。

背景信息

Phospho-TOPBP1(S1138) antibody detects the phosphorylation of TOPBP1 (DNA Topoisomerase IIβ-binding protein 1) at serine 1138. a post-translational modification critical for its role in DNA damage response (DDR) pathways. TOPBP1 is a multi-functional scaffold protein involved in ATR (ataxia-telangiectasia and Rad3-related kinase)-mediated checkpoint signaling, replication stress resolution, and DNA repair. Phosphorylation at S1138 occurs in response to DNA damage, particularly during replication stress or double-strand breaks, and is thought to facilitate TOPBP1’s interaction with components of the DDR machinery, such as ATR-ATRIP complexes, to amplify checkpoint signaling and stabilize stalled replication forks. This modification enhances TOPBP1’s ability to activate ATR, promoting cell cycle arrest and allowing time for DNA repair. The Phospho-TOPBP1(S1138) antibody is widely used in research to study DDR dynamics, replication stress, and cancer biology, as dysregulation of these pathways is linked to genomic instability and tumorigenesis. It is validated for applications like Western blotting, immunofluorescence, and immunohistochemistry to assess TOPBP1 activation status in cell lines or tissue samples exposed to genotoxic agents (e.g., UV, ionizing radiation, or chemotherapeutics). Proper controls, including phosphatase treatment or non-phosphorylatable mutants, are recommended to confirm specificity. Understanding TOPBP1 phosphorylation provides insights into cellular responses to genomic stress and therapeutic strategies targeting DDR-deficient cancers.

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