| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nuclear protein localization protein 4 homolog, Protein NPL4, NPLOC4, KIAA1499, NPL4 |
| Entrez GeneID | 55666 |
| WB Predicted band size | 68.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NPLOC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-484 amino acids from the C-terminal region of human NPLOC4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下为模拟生成的参考文献示例(实际文献需通过学术数据库验证)：
1. **文献名称**: "NPLOC4 as a component of the E3 ubiquitin ligase complex in ER-associated degradation"
**作者**: Yamamoto S, et al.
**摘要**: 研究揭示了NPLOC4作为E3泛素连接酶复合物的关键亚基，参与内质网相关蛋白降解(ERAD)过程。通过NPLOC4抗体进行免疫共沉淀实验，证实其与HRD1等蛋白的相互作用，调控错误折叠蛋白的泛素化及蛋白酶体降解。
2. **文献名称**: "NPLOC4 modulates viral immune evasion via ubiquitination of host restriction factors"
**作者**: Chen L, et al.
**摘要**: 本文利用NPLOC4抗体进行Western blot和免疫荧光定位，发现其在病毒感染中通过泛素化修饰宿主抗病毒蛋白(如IFITM3)，影响病毒颗粒的组装与释放，为病毒免疫逃逸机制提供新见解。
3. **文献名称**: "Functional characterization of NPLOC4 in cell cycle regulation"
**作者**: Gupta R, et al.
**摘要**: 通过RNA干扰和NPLOC4抗体介导的蛋白检测，研究发现NPLOC4通过调控Cyclin D1的稳定性影响G1/S期转换，其缺失导致细胞周期停滞及凋亡增加，提示其在肿瘤发生中的潜在作用。
4. **文献名称**: "Proteomic analysis of NPLOC4-associated complexes in cancer cells"
**作者**: Wang H, et al.
**摘要**: 采用NPLOC4抗体进行亲和纯化-质谱分析，鉴定出多种与癌症代谢相关的互作蛋白(如VCP/p97)，揭示NPLOC4通过调控线粒体蛋白稳态参与肿瘤细胞耐药的分子机制。
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**说明**：以上为基于NPLOC4已知功能(泛素化、ERAD、病毒互作等)模拟的文献示例，实际研究需查阅PubMed、Google Scholar等平台(关键词：NPLOC4 antibody, USP50. E3 ligase)。建议结合具体研究方向筛选文献。
The NPLOC4 antibody targets the NPL4 homolog, encoded by the *NPLOC4* gene, a critical component of the ubiquitin-proteasome system. NPLOC4 forms a complex with UFD1 and the ATPase valosin-containing protein (VCP/p97), facilitating endoplasmic reticulum-associated degradation (ERAD). This process identifies and disposes misfolded proteins, maintaining cellular proteostasis. The NPLOC4-UFD1-p97 complex extracts polyubiquitinated substrates from membranes or macromolecular assemblies, enabling their proteasomal degradation. Dysregulation of this pathway is linked to neurodegenerative diseases, cancer, and inclusion body myopathies.
Antibodies against NPLOC4 are essential tools for studying ERAD mechanisms, protein quality control, and VCP/p97-associated pathologies. They are used in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to assess NPLOC4 expression, localization, and interactions. Researchers also employ these antibodies to explore NPLOC4's role in cell cycle regulation, DNA repair, and viral infection responses. Commercial NPLOC4 antibodies are typically raised in rabbits or mice, validated for specificity across human, mouse, and rat samples.
Understanding NPLOC4's function through antibody-based assays provides insights into diseases caused by proteostatic dysfunction, aiding therapeutic development targeting the ubiquitin-proteasome pathway.
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