| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Mitochondrial Rho GTPase 2, MIRO-2, hMiro-2, 365-, Ras homolog gene family member T2, RHOT2, ARHT2, C16orf39 |
| Entrez GeneID | 89941 |
| WB Predicted band size | 68.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RHOT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-320 amino acids from the Central region of human RHOT2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RHOT2抗体的3篇参考文献示例(注：文献信息为模拟概括，实际引用需核实原文)：
1. **"RHOT2 regulates mitochondrial dynamics and apoptosis in neurons"**
*作者：C. Fröhlich et al. (2017)*
**摘要**：研究利用特异性RHOT2抗体，揭示其在神经元线粒体融合-分裂平衡中的作用，并发现RHOT2缺失导致线粒体功能障碍和细胞凋亡。
2. **"Mitochondrial Rho GTPase RHOT2 interacts with MIRO1 to control mitochondrial trafficking"**
*作者：M. Sato et al. (2014)*
**摘要**：通过免疫共沉淀(使用RHOT2抗体)证实RHOT2与MIRO1的相互作用，阐明二者协同调控线粒体在细胞内的定向运输机制。
3. **"Aberrant RHOT2 expression in cancer cells promotes metabolic reprogramming"**
*作者：L. Huang et al. (2019)*
**摘要**：采用RHOT2抗体进行免疫组化分析，发现其在多种癌症中高表达，并通过激活线粒体氧化磷酸化驱动肿瘤代谢适应性改变。
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**提示**：RHOT2是线粒体外膜Rho GTP酶，参与线粒体动态及运输。如需精准文献，建议在PubMed中以“RHOT2 antibody”或“RHOT2 mitochondrial transport”为关键词检索，并筛选涉及抗体开发或功能性研究的论文。
**Background of RHOT2 Antibody**
The RHOT2 antibody targets the mitochondrial Rho GTPase 2 (RHOT2/MIRO2), a protein encoded by the *RHOT2* gene in humans. RHOT2 is a key regulator of mitochondrial dynamics and intracellular transport, functioning as a calcium-sensitive adaptor that links mitochondria to kinesin or dynein motor proteins via trafficking complexes. This interaction enables mitochondria to move along microtubules, ensuring proper distribution to regions with high metabolic demands, such as neuronal synapses or dividing cells.
RHOT2 contains two GTPase domains and two calcium-binding EF-hand motifs, allowing it to coordinate mitochondrial motility with cellular energy needs and calcium signaling. Dysregulation of RHOT2 is implicated in neurodegenerative diseases (e.g., Parkinson’s and Alzheimer’s) and cancer, where altered mitochondrial trafficking contributes to apoptosis resistance or metabolic reprogramming.
RHOT2 antibodies are essential tools for studying mitochondrial behavior, cellular stress responses, and disease mechanisms. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess RHOT2 expression, localization, and interactions. Many commercial RHOT2 antibodies are raised in rabbits or mice against synthetic peptides corresponding to conserved regions of the human protein. Validation typically includes knockout cell lines or tissue-specific expression patterns to confirm specificity. Recent studies also utilize RHOT2 antibodies to explore its role in mitophagy, ER-mitochondria contacts, and therapeutic targeting in cancer.
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