| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase TBK1, T2K, TANK-binding kinase 1, Tbk1 |
| Entrez GeneID | 56480 |
| WB Predicted band size | 83.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Tbk1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-35 amino acids from the N-terminal region of mouse Tbk1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与小鼠TBK1(N端)抗体相关的参考文献摘要示例：
1. **文献名称**: "TBK1 is essential for innate immunity to RNA viruses"
**作者**: Ishikawa, H. et al.
**摘要**: 研究利用针对小鼠TBK1 N端的抗体，通过免疫沉淀和Western blot验证了TBK1在RNA病毒(如VSV)感染中激活I型干扰素通路的关键作用，揭示了TBK1在巨噬细胞中的功能依赖性。
2. **文献名称**: "Antibody-based profiling of TBK1 phosphorylation in autoimmune disorders"
**作者**: Li, X. et al.
**摘要**: 通过N端特异性TBK1抗体，分析了系统性红斑狼疮(SLE)小鼠模型中的TBK1磷酸化水平，证明TBK1异常激活与自身免疫反应的关联，抗体用于组织切片染色及流式细胞术。
3. **文献名称**: "Development and validation of a panel of TBK1-specific monoclonal antibodies"
**作者**: Chen, Z. et al.
**摘要**: 报道了针对小鼠TBK1 N端表位的单克隆抗体制备，通过ELISA、免疫组化及敲除细胞系验证抗体特异性，确认其在检测内源性TBK1表达及亚细胞定位中的应用。
4. **文献名称**: "TBK1 regulates metabolic homeostasis in adipose tissue via NF-κB signaling"
**作者**: Chen, Y. et al.
**摘要**: 使用TBK1 N端抗体研究肥胖小鼠模型中TBK1的蛋白表达变化，发现其通过调控脂肪组织炎症影响胰岛素敏感性，抗体用于Western blot及免疫荧光共定位分析。
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注：上述文献为模拟概括，实际引用需通过PubMed等平台检索具体论文(可结合关键词“TBK1 antibody N-term mouse”或“TBK1 kinase immune signaling”查找)。
The Mouse Tbk1 (N-term) antibody is designed to specifically detect the N-terminal region of TANK-binding kinase 1 (TBK1) in mouse samples. TBK1. a member of the IκB kinase (IKK) family, plays a critical role in innate immunity, autophagy, and inflammatory signaling pathways. It acts as a key regulator of type I interferon (IFN) production by phosphorylating transcription factors like IRF3 and IRF7. which drive antiviral and antibacterial responses. TBK1 is also involved in selective autophagy through interactions with autophagy adaptors such as optineurin and p62. Dysregulation of TBK1 has been linked to autoimmune disorders, viral infections, and cancer.
This antibody targets the N-terminal domain of mouse TBK1. enabling researchers to study its expression, post-translational modifications, and activation status in various biological contexts. It is commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate TBK1's role in signaling pathways such as STING, TLR, or RIG-I-mediated immune responses. Validation typically includes testing on TBK1-deficient cells or tissues to confirm specificity. Researchers employ this tool to explore TBK1's involvement in disease mechanisms or therapeutic targeting, particularly in models of neuroinflammation, viral infection, and tumor immunity.
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