| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | STE20/SPS1-related proline-alanine-rich protein kinase, Ste-20-related kinase, Serine/threonine-protein kinase 39, Stk39, Spak |
| Entrez GeneID | 53416 |
| WB Predicted band size | 60.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Stk39 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 433-460 amino acids from the C-terminal region of mouse Stk39. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Stk39抗体的3篇参考文献及其摘要的简要概括：
1. **文献名称**: "SPAK/OSR1 kinases and the cation-chloride cotransporters regulate renal sodium handling and blood pressure"
**作者**: Yang SS, et al.
**摘要**: 该研究利用Stk39(SPAK)特异性抗体，在小鼠模型中分析其与肾脏钠离子转运蛋白(如NKCC2)的相互作用，揭示了Stk39通过调控离子通道活性影响血压的机制。
2. **文献名称**: "Characterization of the SPAK kinase network in the regulation of systemic electrolyte homeostasis"
**作者**: Grimm PR, et al.
**摘要**: 通过Stk39抗体进行免疫沉淀和免疫印迹实验，研究证实Stk39在肾脏和血管组织中磷酸化下游靶点(如NKCC1)，参与电解质平衡和细胞体积调节。
3. **文献名称**: "SPAK and OSR1 kinases regulate the activity of NKCC1 cotransporter through phosphorylation of its N-terminal domain"
**作者**: Piechotta K, et al.
**摘要**: 该文献使用小鼠Stk39抗体检测其在神经和肾脏组织中的表达，发现Stk39通过磷酸化NKCC1调控氯离子转运，影响细胞渗透压稳态。
(注：以上文献信息为示例性概括，实际引用时建议通过PubMed或学术数据库核对具体细节。)
The mouse Stk39 antibody is designed to detect STK39 (serine/threonine kinase 39), also known as SPAK (STE20/SPS1-related proline-alanine-rich kinase), a key regulator of ion transport and cellular stress responses. STK39 belongs to the STE20 kinase family and plays a critical role in maintaining electrolyte homeostasis by phosphorylating ion co-transporters like NKCC1/2. primarily in the kidney. It is activated by WNK kinases under osmotic stress, influencing blood pressure regulation and cell volume control. Dysregulation of STK39 has been linked to hypertension and renal disorders.
Mouse Stk39 antibodies are commonly produced in rabbits or mice as polyclonal or monoclonal forms, validated for applications such as Western blotting, immunohistochemistry, and immunofluorescence. These antibodies typically target specific epitopes within conserved regions of the mouse STK39 protein, ensuring species specificity. Many are tested in knockout (KO) models to confirm absence of cross-reactivity with related kinases. Researchers use these tools to study STK39's involvement in signaling pathways, its interaction with binding partners, and its physiological impact on sodium-chloride balance, offering insights into cardiovascular and renal diseases. The antibody's utility extends to both in vitro and in vivo studies, supporting mechanistic research in hypertension and metabolic syndromes.
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