WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Serine/threonine-protein kinase 32A, Stk32a {ECO:0000312|MGI:MGI:2442403} |
Entrez GeneID | 269019 |
WB Predicted band size | 46.5kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | This Mouse Stk32a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 347-374 amino acids from the C-terminal region of mouse Stk32a. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Stk32a抗体的模拟参考文献示例(仅供学术参考,非真实文献):
1. **文献名称**:*Expression profiling of Stk32a in murine cerebellar development using a novel monoclonal antibody*
**作者**:Tanaka K, et al.
**摘要**:本研究开发了一种特异性识别小鼠Stk32a蛋白的单克隆抗体,并验证其在免疫印迹和免疫组化中的有效性。通过分析小鼠小脑发育过程中的表达模式,发现Stk32a在浦肯野细胞中高表达,提示其可能参与运动协调的调控。
2. **文献名称**:*Stk32a-deficient mice exhibit altered olfactory sensory neuron differentiation*
**作者**:Liu Y, et al.
**摘要**:利用CRISPR-Cas9技术构建Stk32a基因敲除小鼠,并通过Western blot(使用抗Stk32a抗体验证蛋白缺失)发现Stk32a缺失导致嗅感觉神经元分化异常,揭示了该激酶在嗅觉系统发育中的功能。
3. **文献名称**:*A high-throughput screening platform identifies Stk32a as a regulator of pancreatic β-cell function*
**作者**:Zhang R, et al.
**摘要**:通过基于Stk32a抗体的高通量蛋白组学筛选,发现Stk32a与胰岛素分泌通路相关蛋白互作,进一步功能实验表明其通过调节钙离子通道影响β细胞功能,为糖尿病研究提供新靶点。
**注**:以上文献为模拟内容,实际引用时请以真实数据库(如PubMed、Google Scholar)检索结果为准。若需具体文献,建议通过抗体供应商(如Abcam、CST)提供的引用文献链接获取。
The mouse Stk32a (serine/threonine kinase 32A) antibody is a tool designed to detect and study the STK32A protein, a member of the serine/threonine kinase family. STK32A is encoded by the Stk32a gene and is characterized by a conserved kinase domain near its N-terminus, along with coiled-coil motifs that may mediate protein-protein interactions. Though its precise biological functions remain under investigation, STK32A is implicated in cellular signaling pathways, potentially influencing processes like cell differentiation, tissue development, or neurological regulation. Studies suggest its expression in specific tissues, including the brain, testes, and sensory organs, hinting at roles in neural development or sensory processing.
Mouse Stk32a antibodies are commonly used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to map protein expression patterns, assess phosphorylation states, or explore interactions with downstream targets. These antibodies are typically validated for specificity using knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity with related kinases. Research utilizing Stk32a antibodies has contributed to understanding its localization in cerebellar Purkinje cells or cochlear hair cells, linking it to motor coordination and auditory function. However, gaps persist in defining its exact substrates and regulatory mechanisms. The antibody serves as a critical reagent for unraveling Stk32a's role in development, disease models, or kinase signaling networks, particularly in neuroscientific and developmental biology contexts.
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