| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein Hox-B5, Homeobox protein H241, Homeobox protein Hox-21, Homeobox protein Mu-1, Hoxb5, Hox-21, Hoxb-5 |
| Entrez GeneID | 15413 |
| WB Predicted band size | 29.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Hoxb5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of mouse Hoxb5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及Mouse Hoxb5(N-term)抗体的文献摘要信息(注：部分文献可能为示例性描述，需根据实际数据库验证)：
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1. **文献名称**：*Hoxb5 regulates endothelial progenitor cell differentiation in mouse embryonic development*
**作者**：Zhang Y. et al.
**摘要**：研究Hoxb5在小鼠胚胎内皮祖细胞分化中的作用，使用N端特异性抗体通过免疫荧光和Western blot验证Hoxb5蛋白在早期胚胎中的表达模式及功能调控。
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2. **文献名称**：*Spatiotemporal expression of Hoxb5 in the developing mouse lung*
**作者**：Li Q. & Chen H.
**摘要**：通过针对Hoxb5 N端的多克隆抗体进行免疫组化分析，揭示Hoxb5在小鼠肺发育不同阶段的动态表达，提示其参与肺泡上皮细胞分化的分子机制。
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3. **文献名称**：*Hoxb5 modulates neural crest cell migration in the enteric nervous system*
**作者**：Smith J.R. et al.
**摘要**：利用Hoxb5(N-term)特异性抗体阻断实验，证明Hoxb5通过调控Wnt信号通路影响神经嵴细胞迁移，研究为肠道神经系统发育提供了新见解。
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**注意事项**：
- 以上文献为示例性内容，实际引用需通过PubMed、Web of Science等数据库检索真实文献。
- 推荐补充关键词搜索：`"Hoxb5 antibody" + mouse + N-terminal`，并关注抗体供应商(如Abcam、Santa Cruz)提供的引用文献列表。
The Mouse Hoxb5 (N-term) antibody is a specialized tool designed to detect the N-terminal region of the Hoxb5 protein, a member of the homeobox (Hox) gene family. Hox genes encode transcription factors critical for embryonic development, particularly in establishing body segmentation and anterior-posterior patterning. HOXB5. part of the HoxB cluster on chromosome 11. plays roles in thoracic vertebral development, neural crest cell migration, and organogenesis, including lung and heart formation. The antibody targets the N-terminal domain, which is involved in protein-protein interactions and regulatory functions, distinct from the conserved homeodomain responsible for DNA binding.
This antibody is commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study HOXB5 expression and localization in murine tissues. Its specificity for the N-terminal region helps differentiate HOXB5 from other Hox proteins with similar homeodomains, though cross-reactivity with paralogs sharing sequence homology (e.g., HOXA5 or HOXC5) should be validated experimentally. Researchers employ it to investigate HOXB5's role in developmental biology, stem cell differentiation, and disease models, particularly cancers where HOX genes are often dysregulated. For example, HOXB5 has been implicated in leukemia progression and solid tumor metastasis. Proper controls, including knockout models or peptide-blocking assays, are recommended to confirm signal specificity. Commercial versions are typically developed in rabbit or goat hosts, with validation data provided for key applications.
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