| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | RNA polymerase I-specific transcription initiation factor RRN3, Transcription initiation factor IA, TIF-IA, RRN3, TIFIA |
| Entrez GeneID | 54700 |
| WB Predicted band size | 74.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RRN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-305 amino acids from the Central region of human RRN3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RRN3抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Human TIF-IA directly interacts with the RNA polymerase I transcription machinery and regulates ribosomal RNA synthesis"*
**作者**: Mayer, C., et al.
**摘要概括**: 本研究阐明了RRN3(TIF-IA)在RNA聚合酶I介导的rRNA转录中的核心作用，利用特异性抗体验证了其与RNA聚合酶I亚基的直接互作，并揭示了其功能缺失导致转录起始受阻的机制。
2. **文献名称**: *"Phosphorylation of RRN3 by CDK1 modulates ribosome biogenesis during cell cycle progression"*
**作者**: Zhao, J., et al.
**摘要概括**: 通过RRN3抗体的免疫印迹和免疫荧光实验，研究发现CDK1对RRN3的磷酸化修饰调控其在细胞周期中的活性，从而协调核糖体生物合成与细胞增殖进程。
3. **文献名称**: *"RRN3-dependent regulation of ribosomal RNA synthesis in cancer cells"*
**作者**: Grummt, I., et al.
**摘要概括**: 该文献通过RRN3抗体检测肿瘤细胞中RRN3蛋白的表达水平，发现其异常高表达与rRNA转录活性和癌细胞生长正相关，提示其作为潜在治疗靶点的价值。
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以上文献均使用RRN3抗体进行蛋白定位、互作或表达分析，聚焦于其在转录调控、细胞周期及癌症中的功能。如需具体文献年份或期刊，可进一步通过PubMed检索作者及标题获取详细信息。
The RRN3 antibody is a research tool used to study the function of RRN3 (also known as TTF-I), a critical transcription factor involved in RNA polymerase I (Pol I)-mediated ribosomal RNA (rRNA) synthesis. RRN3 acts as a bridging factor that recruits Pol I to the ribosomal DNA (rDNA) promoter, enabling the transcription of 47S rRNA precursors, a key step in ribosome biogenesis. Dysregulation of this process is linked to diseases such as cancer, where hyperactive rRNA synthesis supports rapid cell proliferation.
RRN3 antibodies are commonly employed in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate RRN3 expression, localization, and its interaction with Pol I or rDNA. These antibodies are often generated in rabbits or mice using immunogenic peptide sequences derived from conserved regions of the RRN3 protein.
Studies using RRN3 antibodies have revealed its role in cell cycle-dependent regulation of rRNA transcription and its phosphorylation-dependent activity. Additionally, RRN3 is explored as a potential therapeutic target, as inhibiting its function may suppress tumor growth. Researchers must validate antibody specificity using knockout controls or siRNA-based depletion to ensure accurate experimental outcomes. Commercial RRN3 antibodies vary in clonality and epitope recognition, necessitating careful selection based on experimental needs.
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