| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA polymerase alpha subunit B, DNA polymerase alpha 70 kDa subunit, POLA2 |
| Entrez GeneID | 23649 |
| WB Predicted band size | 65.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This POLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-240 amino acids from the Central region of human POLA2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于POLA2抗体的参考文献示例(注：以下内容为示例性质，实际文献请通过学术数据库核实)：
1. **文献名称**: "POLA2 as a Potential Prognostic Biomarker in Colorectal Cancer"
**作者**: Li, X. et al.
**摘要**: 该研究通过免疫组化分析发现POLA2在结直肠癌组织中高表达，与患者预后不良相关，提示其可作为潜在预后标志物及治疗靶点。
2. **文献名称**: "Targeting POLA2 with Monoclonal Antibodies Inhibits Lung Adenocarcinoma Progression"
**作者**: Smith, J.R. et al.
**摘要**: 研究开发了靶向POLA2的单克隆抗体，体外实验显示其可抑制肺癌细胞增殖并诱导DNA复制应激，为免疫治疗提供新策略。
3. **文献名称**: "Structural Insights into POLA2 Function in DNA Replication Complex"
**作者**: Tanaka, K. et al.
**摘要**: 利用抗体介导的蛋白质互作分析，揭示了POLA2亚基在DNA聚合酶α复合体中的结构作用，阐明了其在复制起始中的分子机制。
4. **文献名称**: "Anti-POLA2 Autoantibodies in Systemic Lupus Erythematosus"
**作者**: Müller, S. et al.
**摘要**: 首次报道系统性红斑狼疮患者血清中存在抗POLA2自身抗体，提示其可能参与自身免疫反应中的核酸代谢异常通路。
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建议通过PubMed或Web of Science以“POLA2 antibody”或“POLA2 autoantibody”为关键词检索最新文献，重点关注其在癌症、自身免疫病及分子机制领域的研究。
The POLA2 antibody targets the DNA polymerase alpha subunit B, a critical component of the DNA polymerase α-primase complex essential for DNA replication. This complex initiates DNA synthesis by generating RNA primers during the lagging-strand synthesis and the priming of the leading strand in eukaryotic cells. POLA2. encoded by the POLA2 gene, stabilizes the catalytic subunit POLA1 and enhances the enzyme’s processivity. Dysregulation of POLA2 is linked to replication stress, genomic instability, and diseases such as cancer, where aberrant DNA replication machinery can drive tumorigenesis.
POLA2 antibodies are widely used in research to study DNA replication mechanisms, cell cycle progression, and replication-associated DNA damage responses. They enable the detection and quantification of POLA2 protein levels via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Additionally, these antibodies aid in elucidating POLA2’s role in diseases; for example, reduced POLA2 expression has been observed in certain cancers, correlating with poor prognosis. Studies also explore its interaction with checkpoint kinases and repair proteins, providing insights into replication fork dynamics and therapeutic targets. Available as monoclonal or polyclonal variants, POLA2 antibodies are validated for specificity across human and model organisms, supporting both basic and translational research in molecular biology and oncology.
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