| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein phosphatase methylesterase 1, PME-1, PPME1, PME1 |
| Entrez GeneID | 51400 |
| WB Predicted band size | 42.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PPME1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 351-379 amino acids from the C-terminal region of human PPME1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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**Background of PPME1 Antibody**
PPME1 (Protein Phosphatase Methylesterase 1), also known as PME-1. is a critical enzyme involved in the post-translational regulation of protein phosphatase 2A (PP2A), a major serine/threonine phosphatase essential for cellular signaling. PPME1 specifically demethylates the conserved leucine 309 residue on the PP2A catalytic subunit (PP2Ac), reversing methyl-esterification mediated by the PP2A methyltransferase LCMT1. This dynamic methylation-demethylation cycle regulates PP2A’s holoenzyme assembly, substrate specificity, and subcellular localization, thereby influencing diverse processes such as cell cycle progression, apoptosis, and stress responses.
PPME1 antibodies are valuable tools for studying PP2A regulation and its implications in disease. Dysregulation of PPME1 has been linked to cancer, neurodegenerative disorders (e.g., Alzheimer’s), and metabolic syndromes, often due to altered PP2A activity. Researchers use PPME1 antibodies in techniques like Western blotting, immunoprecipitation, and immunohistochemistry to quantify protein expression, assess interactions with PP2A subunits, or map tissue-specific distribution.
Structurally, PPME1 contains a conserved metallo-β-lactamase fold critical for its methylesterase activity. Its N-terminal catalytic domain and C-terminal flexible regions are often epitope targets for antibodies. Studies using PPME1-specific antibodies have highlighted its oncogenic role in stabilizing cancer-associated PP2Ac mutants and promoting tumor growth, making it a potential therapeutic target. Understanding PPME1’s function through antibody-based assays continues to shed light on PP2A biology and disease mechanisms.
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