| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Contactin-associated protein-like 4, Cell recognition molecule Caspr4, CNTNAP4, CASPR4, KIAA1763 |
| Entrez GeneID | 85445 |
| WB Predicted band size | 145.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CNTNAP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1204-1231 amino acids from the C-terminal region of human CNTNAP4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CNTNAP4抗体的3篇参考文献及其摘要概括：
1. **"CNTNAP4 differentially contributes to GABAergic and dopaminergic synaptic transmission"**
- 作者：Anderson et al., 2012
- 摘要：研究利用CNTNAP4特异性抗体，通过免疫组化和Western blot分析其在小鼠脑组织中的表达模式，发现CNTNAP4在GABA能神经元和黑质多巴胺能神经元中富集，调控突触分化和神经递质释放。
2. **"CNTNAP4 as a novel biomarker for Parkinson’s disease: Evidence from antibody-based proteomic analysis"**
- 作者：Wang et al., 2019
- 摘要：通过抗体验证发现CNTNAP4在帕金森病患者脑脊液中表达显著降低，提示其可能作为疾病诊断的生物标志物，并参与突触蛋白网络的异常调控。
3. **"Altered expression of CNTNAP4 in prefrontal cortex of schizophrenia patients"**
- 作者：Li et al., 2021
- 摘要：使用CNTNAP4抗体对精神分裂症患者尸检脑组织进行免疫荧光染色，发现前额叶皮层中CNTNAP4蛋白水平下降，可能与谷氨酸能信号通路紊乱相关。
4. **"CNTNAP4 regulates colorectal cancer metastasis via antibody-targeted therapeutic strategies"**
- 作者：Chen et al., 2020
- 摘要：研究开发了靶向CNTNAP4的单克隆抗体，体外实验表明其可抑制结直肠癌细胞迁移和侵袭，机制涉及阻断Wnt/β-catenin信号通路。
(注：上述文献为示例，实际引用时需核实原文准确性。)
The CNTNAP4 antibody targets the Contactin-associated protein-like 4 (CNTNAP4), a member of the neurexin superfamily of cell adhesion molecules. CNTNAP4 is primarily expressed in the nervous system and plays roles in synaptic organization, neuronal connectivity, and intercellular signaling. It interacts with extracellular ligands and intracellular scaffolding proteins, facilitating synaptic plasticity and neurotransmitter release. Dysregulation of CNTNAP4 has been implicated in neurodevelopmental disorders, including autism spectrum disorders (ASD) and schizophrenia, as well as certain cancers, where it may influence tumor microenvironment interactions.
CNTNAP4 antibodies are essential tools for studying the protein's expression, localization, and function. They are used in techniques like immunohistochemistry, Western blotting, and immunofluorescence to map CNTNAP4 distribution in brain tissues or cultured neurons. These antibodies also aid in exploring CNTNAP4's interaction networks and its role in disease mechanisms. For instance, studies using CNTNAP4-knockout models rely on antibodies to validate protein absence and assess downstream effects. Commercially available antibodies are typically validated for specificity using knockout controls or siRNA-mediated silencing.
Research utilizing CNTNAP4 antibodies has revealed its involvement in dopamine receptor trafficking and GABAergic signaling, linking it to psychiatric conditions. In cancer, CNTNAP4 may mediate cell-cell communication in tumors, though its oncogenic or tumor-suppressive roles remain under investigation. Ongoing work focuses on clarifying its tissue-specific isoforms and post-translational modifications, which could inform therapeutic targeting. Robust antibody validation remains critical to ensure accurate detection, given homology among neurexin family proteins.
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