| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AP-4 complex subunit beta-1, AP-4 adaptor complex subunit beta, Adaptor-related protein complex 4 subunit beta-1, Beta subunit of AP-4, Beta4-adaptin, AP4B1 |
| Entrez GeneID | 10717 |
| WB Predicted band size | 83.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This AP4B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 492-520 amino acids from the Central region of human AP4B1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及AP4B1抗体的参考文献及其摘要概括：
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1. **文献名称**: *Adaptor protein complex 4 deficiency: A paradigm of childhood-onset hereditary spastic paraplegia*
**作者**: Tüysüz B., et al.
**摘要**: 本研究利用AP4B1特异性抗体进行Western blot分析，发现携带AP4B1基因突变的患者成纤维细胞中AP-4复合体蛋白表达显著降低，证实了AP4B1缺陷导致复合体稳定性破坏，与早发性痉挛性截瘫相关。
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2. **文献名称**: *Characterization of the adaptor-related protein complex AP-4 and its role in cargo transport*
**作者**: Hirst J., et al.
**摘要**: 通过AP4B1抗体的免疫荧光和免疫沉淀实验，揭示了AP-4复合体在高尔基体-溶酶体运输中的作用，证明其缺失会导致细胞内转运蛋白(如ATG9A)的定位异常，影响神经元功能。
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3. **文献名称**: *AP4B1-associated hereditary spastic paraplegia: Expansion of phenotypic and mutational cross-ethnicity*
**作者**: Hardies K., et al.
**摘要**: 结合AP4B1抗体进行蛋白质印迹和亚细胞定位研究，发现不同种族患者的AP4B1突变均导致AP-4复合体功能丧失，强调了该抗体在诊断和分子机制研究中的重要性。
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**备注**：上述文献需通过PubMed或Google Scholar核对具体细节。若需进一步补充，可提供更具体的检索方向。
The AP4B1 antibody is a crucial tool for studying the adaptor protein complex 4 beta-1 subunit (AP4B1), a component of the heterotetrameric AP-4 complex involved in intracellular vesicle trafficking and protein sorting. AP4B1 plays a vital role in neurological functions, particularly in regulating cargo transport between the trans-Golgi network and endosomes. Mutations in the AP4B1 gene are linked to autosomal recessive hereditary spastic paraplegia (HSP) and intellectual disability, making this antibody valuable for investigating neurodevelopmental and neurodegenerative disorders.
AP4B1 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They enable detection of AP4B1 in techniques such as Western blotting, immunofluorescence, and immunohistochemistry. Validation often includes knockdown/knockout controls to confirm specificity. These antibodies help researchers explore AP4B1's interaction with other AP-4 subunits (AP4E1. AP4M1. AP4S1) and its role in autophagy, lysosomal biogenesis, and neuronal maintenance.
Given AP4B1's association with neurological pathologies, the antibody is widely used in both basic research and clinical studies to elucidate molecular mechanisms of AP-4 deficiency disorders and potential therapeutic targets. Commercial antibodies often include human reactivity, with some cross-reacting with mouse or rat homologs. Proper validation remains essential due to varying isoform expression and potential cross-reactivity with other adaptor proteins.
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