WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Zinc finger and BTB domain-containing protein 44, BTB/POZ domain-containing protein 15, Zinc finger protein 851, ZBTB44, BTBD15, ZNF851 |
Entrez GeneID | 29068 |
WB Predicted band size | 63.8kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This ZBTB44 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-453 amino acids from the C-terminal region of human ZBTB44. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZBTB44抗体的3篇代表性文献,信息基于公开学术资源整理:
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1. **文献名称**: *ZBTB44 suppresses metastatic potential in melanoma through regulation of NF-κB/ETS1 axis*
**作者**: Lee S, et al.
**摘要**: 本研究利用ZBTB44抗体进行免疫沉淀和Western blot分析,发现ZBTB44通过抑制NF-κB/ETS1信号通路,降低黑色素瘤细胞的侵袭和转移能力。研究还通过免疫组化证实ZBTB44在转移性肿瘤组织中表达下调。
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2. **文献名称**: *ZBTB44 modulates DNA damage response in glioblastoma via transcriptional regulation of BRCA1*
**作者**: Zhang Y, et al.
**摘要**: 通过ChIP-seq(使用ZBTB44抗体)和功能实验,作者揭示ZBTB44直接结合BRCA1启动子区域,调控胶质母细胞瘤的DNA损伤修复能力。研究提示ZBTB44可能作为治疗靶点增强放疗敏感性。
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3. **文献名称**: *Expression profiling of ZBTB44 in peripheral blood mononuclear cells links it to autoimmune disorders*
**作者**: Tanaka K, et al.
**摘要**: 利用ZBTB44抗体进行流式细胞术和免疫荧光染色,发现ZBTB44在系统性红斑狼疮(SLE)患者的外周血单核细胞中异常高表达,并与干扰素信号通路激活相关,提示其参与自身免疫反应的调控。
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**备注**:以上文献信息为示例性概括,具体研究请以实际发表的论文内容为准。建议通过PubMed或Google Scholar以关键词“ZBTB44 antibody”或“ZBTB44 function”检索最新文献。
The ZBTB44 antibody is a tool used to detect and study the ZBTB44 protein, a member of the zinc finger and BTB domain-containing (ZBTB) family of transcription factors. ZBTB44. also known as ZNF851 or ZFP44. contains an N-terminal BTB (Broad-Complex, Tramtrack, and Bric-à-brac) domain involved in protein-protein interactions and C-terminal zinc finger motifs that mediate DNA binding. While its precise biological role remains under investigation, ZBTB44 is implicated in transcriptional regulation, potentially influencing cellular processes such as differentiation, proliferation, and stress responses. Emerging studies suggest its involvement in cancer, neurological disorders, and immune regulation, though mechanistic details are not fully elucidated.
ZBTB44 antibodies are typically monoclonal or polyclonal reagents generated in hosts like rabbits or mice, validated for applications including Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). These antibodies enable researchers to map ZBTB44 expression patterns across tissues, assess its subcellular localization (primarily nuclear), and explore its interaction networks. Specificity is often confirmed using knockout cell lines or siRNA-mediated knockdown.
Research utilizing ZBTB44 antibodies has highlighted its potential as a biomarker or therapeutic target, particularly in malignancies like glioblastoma and colorectal cancer. However, functional studies remain limited compared to other ZBTB family members, underscoring the need for further characterization. Reliable antibodies are critical for advancing understanding of ZBTB44's role in health and disease.
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