| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein Hox-A2, Homeobox protein Hox-111, Hox111, Hoxa2, Hox-111, Hoxa-2 |
| Entrez GeneID | 15399 |
| WB Predicted band size | 40.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Hoxa2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-217 amino acids from the Central region of mouse Hoxa2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及小鼠Hoxa2抗体的关键文献概览：
1. **"Hoxa2 Mutant Mice Exhibit Homeotic Transformation of Skeletal Elements Derived from Cranial Neural Crest"**
- Gendron-Maguire, M., et al. (1993), *Cell*
- 首次通过Hoxa2抗体定位发现该基因在胚胎颅神经嵴中的表达模式，揭示其控制第二鳃弓骨骼发育的调控机制。
2. **"Hoxa2 Knockout Mice Show Developmental Arrest of Middle Ear Ossicles"**
- Rijli, F.M., et al. (1993), *Cell*
- 采用Hoxa2抗体进行胚胎组织染色，证实该基因缺失导致中耳听小骨发育异常，阐明其对颅面骨形态的特异性调控。
3. **"Regulation of Branchiomotor Neuron Migration by Hoxa2"**
- Arenkiel, B.R., et al. (2004), *Developmental Biology*
- 通过抗体介导的蛋白追踪技术，揭示Hoxa2通过SDF1-CXCR4信号通路调控脑干运动神经元迁移的分子机制。
这些研究均通过Hoxa2抗体的免疫组化/荧光技术，解析了该基因在颅面发育、神经嵴分化及神经元迁移中的核心作用。如需全文可通过PMID/DOI在PubMed查询。
The mouse Hoxa2 antibody is a specialized tool used to detect and study the Hoxa2 protein, a member of the Hox gene family critical for embryonic development. Hox genes encode transcription factors that regulate axial patterning and morphogenesis, ensuring proper spatial organization during embryogenesis. Specifically, Hoxa2 plays a pivotal role in craniofacial and hindbrain development. It is expressed in the second pharyngeal arch, influencing the formation of structures such as the hyoid bone, middle ear ossicles, and cranial ganglia. Dysregulation of Hoxa2 is linked to congenital malformations, including craniofacial abnormalities and hearing defects.
The antibody is typically generated by immunizing host animals (e.g., rabbits or mice) with a purified Hoxa2 antigen, such as a peptide fragment unique to the protein. It enables researchers to visualize Hoxa2 expression via techniques like immunohistochemistry, Western blotting, or immunofluorescence. Validated for specificity and sensitivity, the antibody helps map Hoxa2's spatiotemporal expression patterns in tissues, contributing to studies on developmental biology, gene regulatory networks, and disease models.
Its applications extend to investigating Hoxa2's role in stem cell differentiation, tissue regeneration, and evolutionary developmental biology (evo-devo), offering insights into conserved mechanisms across species. By targeting Hoxa2. researchers aim to unravel molecular pathways underlying developmental disorders and potential therapeutic targets.
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