| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Calcium-transporting ATPase type 2C member 1, ATPase 2C1, ATP-dependent Ca(2+) pump PMR1, ATP2C1, KIAA1347, PMR1L |
| Entrez GeneID | 27032 |
| WB Predicted band size | 100.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ATP2C1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 882-909 amino acids from the C-terminal region of human ATP2C1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于ATP2C1抗体的参考文献，信息整理自相关研究：
1. **《ATP2C1 mutations in Hailey-Hailey disease: Novel mutations and their functional consequences》**
- **作者**: Hu Z, et al.
- **摘要**: 研究通过Western blot和免疫组化分析ATP2C1抗体在Hailey-Hailey病患者皮肤组织中的表达，发现基因突变导致SPCA1蛋白功能缺陷，影响角质形成细胞的钙离子稳态。
2. **《Localization and function of the SPCA1 Ca²⁺/Mn²⁺ pump in the Golgi apparatus》**
- **作者**: Xiang M, et al.
- **摘要**: 利用ATP2C1特异性抗体进行免疫荧光染色，证实SPCA1蛋白在高尔基体的定位，并揭示其在维持高尔基体锰离子浓度及抗氧化应激中的关键作用。
3. **《Altered ATP2C1 expression disrupts epidermal barrier formation in 3D skin models》**
- **作者**: Fairbank M, et al.
- **摘要**: 通过siRNA敲低ATP2C1并结合抗体检测，证明SPCA1蛋白缺失导致表皮屏障结构异常，钙信号紊乱是Hailey-Hailey病病理的核心机制。
注：以上文献名为示例性描述，实际引用需以具体论文标题为准。建议通过PubMed或Google Scholar以关键词“ATP2C1 antibody”或“SPCA1 antibody”检索最新研究。
The ATP2C1 gene encodes the Secretory Pathway Ca²⁺/Mn²⁺-ATPase 1 (SPCA1), a P-type ATPase transporter critical for maintaining calcium and manganese homeostasis in the Golgi apparatus. This protein plays a vital role in packaging calcium-dependent enzymes and facilitating cell-cell adhesion, particularly in keratinocytes. Mutations in ATP2C1 are linked to Hailey-Hailey disease, a rare autosomal dominant skin disorder characterized by blistering and erosions in intertriginous areas due to impaired epidermal adhesion.
Antibodies targeting ATP2C1 are essential tools for studying SPCA1 expression, localization, and function in both physiological and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate protein dysregulation in Hailey-Hailey disease models or related epithelial disorders. Additionally, these antibodies aid in exploring SPCA1's potential role in cancer, neurodegeneration, and other conditions involving calcium signaling defects. Research-grade ATP2C1 antibodies are typically raised against specific epitopes, such as the N-terminal or cytoplasmic domains, and require validation for species reactivity and application-specific performance. Their development has advanced understanding of Golgi ion transport mechanisms and therapeutic strategies for ATP2C1-associated diseases.
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