| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Acetylcholine receptor subunit gamma, CHRNG, ACHRG |
| Entrez GeneID | 1146 |
| WB Predicted band size | 57.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CHRNG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-50 amino acids from the N-terminal region of human CHRNG. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于CHRNG(N-term)抗体的参考文献的简要信息：
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1. **文献名称**: "Characterization of monoclonal antibodies against the human acetylcholine receptor γ subunit"
**作者**: Kordeli E, et al.
**摘要**: 该研究开发了针对人源乙酰胆碱受体γ亚基(CHRNG)N端胞外结构域的单克隆抗体，验证了其在免疫印迹和免疫组化中的特异性，并发现该抗体能有效检测胎儿肌肉组织中的CHRNG表达，为先天性肌无力综合征的诊断提供工具。
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2. **文献名称**: "CHRNG-related nonlethal multiple pterygium syndrome: Insights into pathogenesis and antibody reactivity"
**作者**: Vogt J, et al.
**摘要**: 通过Western blot和免疫荧光实验，研究利用CHRNG(N-term)抗体揭示了γ亚基在胚胎神经肌肉接头中的功能异常，证实其突变导致多发性翼状赘肉综合征的分子机制，抗体特异性在患者样本中得到验证。
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3. **文献名称**: "Developmental regulation of nicotinic acetylcholine receptor γ subunit isoforms in human skeletal muscle"
**作者**: Mishina M, et al.
**摘要**: 研究使用CHRNG N端特异性抗体分析γ亚基在胎儿和成人肌肉中的表达差异，发现其在胎儿期高表达，出生后逐渐被ε亚基替代，为神经肌肉接头成熟过程提供分子证据。
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注：上述文献为示例性内容，实际引用需根据具体研究补充完整信息。建议通过PubMed或Google Scholar以关键词“CHRNG antibody N-terminal”检索最新论文。
The CHRNG (N-term) antibody targets the N-terminal region of the gamma subunit of the nicotinic acetylcholine receptor (nAChR), encoded by the CHRNG gene. This subunit is a critical component of the fetal-type neuromuscular junction (NMJ) receptor, primarily expressed during embryonic development. The gamma subunit facilitates acetylcholine binding and ion channel activation, playing a key role in neuromuscular signal transmission. Postnatally, it is replaced by the epsilon subunit (CHRNE), marking the transition to adult NMJ function.
Antibodies against the CHRNG N-terminal domain are often used to study receptor assembly, localization, and developmental regulation. The N-terminal extracellular region is essential for ligand binding and receptor oligomerization, making it a focus for research on neuromuscular disorders. Mutations in CHRNG are linked to Escobar syndrome, a form of multiple pterygium syndrome characterized by congenital joint contractures and skeletal abnormalities. The antibody helps investigate these pathogenic mechanisms by detecting gamma subunit expression in tissues or cell models.
CHRNG (N-term) antibodies are typically validated in applications like Western blotting, immunohistochemistry, and immunofluorescence. Their specificity for the gamma subunit aids in distinguishing fetal from adult receptor isoforms, supporting studies on NMJ maturation or diseases involving defective receptor signaling. Researchers also utilize these antibodies to explore the role of CHRNG in non-neuronal contexts, such as cancer or immune regulation, where nAChR subunits may have atypical functions.
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