| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA-directed RNA polymerase I subunit RPA49, RNA polymerase I subunit A49, DNA-directed RNA polymerase I subunit E, RNA polymerase I-associated factor 1, RNA polymerase I-associated factor 53, POLR1E, PAF53, PRAF1 |
| Entrez GeneID | 64425 |
| WB Predicted band size | 47.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This POLR1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-198 amino acids from the Central region of human POLR1E. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于POLR1E抗体的模拟参考文献(基于领域知识生成，非真实文献)：
1. **文献名称**: "Structural characterization of human RNA polymerase I subunit POLR1E and its antibody interaction"
**作者**: Zhang Y, et al.
**摘要**: 通过X射线晶体学解析POLR1E蛋白的三维结构，并验证商业化抗体(克隆号：AB-2378)在免疫沉淀实验中的特异性，为研究其与核糖体RNA合成的关系提供工具。
2. **文献名称**: "POLR1E expression correlates with poor prognosis in hepatocellular carcinoma"
**作者**: Tanaka K, et al.
**摘要**: 使用抗POLR1E抗体(品牌：CellSign)进行免疫组化分析，发现POLR1E在肝癌组织中高表达，且与患者生存率负相关，提示其作为潜在肿瘤标志物的价值。
3. **文献名称**: "Development of a high-affinity monoclonal antibody against POLR1E for chromatin remodeling studies"
**作者**: Müller S, et al.
**摘要**: 报道一种新型小鼠单克隆抗体(克隆号：mAb-12C4)的制备，通过ChIP-seq验证其在染色质免疫共沉淀中检测POLR1E与rDNA启动子结合的能力。
注：以上为基于领域知识的模拟文献，实际引用时请通过PubMed或Google Scholar检索真实文献。
The POLR1E antibody targets the POLR1E protein (also known as AC19 or RPA49), a subunit of RNA polymerase I (Pol I), which is essential for ribosomal RNA (rRNA) synthesis. Pol I is a multi-subunit enzyme responsible for transcribing the 47S rRNA precursor in the nucleolus, a critical step in ribosome biogenesis and cellular protein synthesis. POLR1E, encoded by the POLR1E gene on human chromosome 14q21.3. interacts with other Pol I components (e.g., RPA194. RPA116) to stabilize the enzyme’s structure and regulate transcription initiation and elongation.
POLR1E antibodies are widely used in research to study Pol I assembly, rRNA transcription dynamics, and nucleolar organization. They are valuable tools for detecting POLR1E expression levels via Western blot (WB), immunofluorescence (IF), or immunohistochemistry (IHC), aiding in investigations of dysregulated ribosome biogenesis in diseases like cancer, developmental disorders, or ribosomopathies. For example, POLR1E mutations have been linked to Treacher Collins syndrome, a craniofacial disorder, and its dysregulation is implicated in tumor proliferation.
These antibodies are typically produced in rabbits or mice, either as monoclonal or polyclonal forms, with validation in specific experimental models (e.g., cell lines, knockout controls). Researchers also use POLR1E antibodies to explore therapeutic targets, as inhibiting Pol I activity may suppress cancer growth. However, cross-reactivity with other Pol subunits or proteins requires careful validation to ensure specificity in experimental settings.
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