| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Prolyl 4-hydroxylase subunit alpha-1, 4-PH alpha-1, Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1, P4HA1, P4HA |
| Entrez GeneID | 5033 |
| WB Predicted band size | 61.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This P4HA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 414-442 amino acids from the C-terminal region of human P4HA1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于P4HA1抗体的3篇示例文献(注：以下内容为模拟文献，实际引用需查询真实数据库)：
1. **文献名称**: "Prolyl 4-hydroxylase subunit alpha 1 (P4HA1) is a biomarker for epithelial-mesenchymal transition and a therapeutic target in cancer"
**作者**: Smith A, et al.
**摘要**: 研究通过免疫组化(IHC)和Western blot分析，发现P4HA1在多种癌症中高表达，并与肿瘤转移相关。使用特异性P4HA1抗体证实其通过调控胶原羟基化促进EMT过程，提示其作为治疗靶点的潜力。
2. **文献名称**: "P4HA1-mediated collagen hydroxylation drives liver fibrosis progression"
**作者**: Chen L, et al.
**摘要**: 本文利用P4HA1抗体在小鼠肝纤维化模型中发现，P4HA1通过促进胶原沉积加剧纤维化。抗体阻断实验结合siRNA敲低表明，抑制P4HA1可减轻纤维化程度，为临床干预提供依据。
3. **文献名称**: "P4HA1 expression correlates with poor prognosis in breast cancer via extracellular matrix remodeling"
**作者**: Wang Y, et al.
**摘要**: 研究通过免疫荧光和流式细胞术分析乳腺癌组织，发现P4HA1高表达患者预后较差。抗体检测显示P4HA1通过调节胶原稳定性促进肿瘤微环境重塑，可能成为预后标志物。
如需具体文献，建议通过PubMed或Google Scholar以关键词“P4HA1 antibody”+“cancer”/“fibrosis”检索近期研究。
P4HA1 (prolyl 4-hydroxylase subunit alpha 1) is a critical enzyme involved in the post-translational modification of collagen, a major structural component of the extracellular matrix. As a subunit of the prolyl 4-hydroxylase complex, P4HA1 catalyzes the hydroxylation of proline residues in collagen chains, a process essential for the stability and triple-helix formation of collagen molecules. This enzymatic activity is vital for maintaining tissue integrity, wound healing, and cellular adhesion. Dysregulation of P4HA1 has been implicated in pathological conditions such as fibrosis, cancer progression, and metastasis, where excessive collagen deposition or remodeling supports tumor microenvironment formation and disease aggressiveness.
Antibodies targeting P4HA1 are valuable tools for studying its expression, localization, and function in both normal and diseased tissues. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein levels in cell lines, clinical samples, or animal models. Recent research highlights P4HA1 as a potential therapeutic target, as its overexpression in cancers correlates with poor prognosis and resistance to therapy. Inhibiting P4HA1 may disrupt collagen maturation, thereby impairing tumor stroma formation or metastatic spread. Studies also explore its role in hypoxic responses, as hydroxylation activity is oxygen-dependent, linking P4HA1 to hypoxia-driven pathways in diseases. Validated P4HA1 antibodies enable mechanistic insights into these processes, supporting drug discovery and biomarker development.
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