| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Endophilin-A2, EEN fusion partner of MLL, Endophilin-2, Extra eleven-nineteen leukemia fusion gene protein, EEN, SH3 domain protein 2B, SH3 domain-containing GRB2-like protein 1, SH3GL1, CNSA1, SH3D2B |
| Entrez GeneID | 6455 |
| WB Predicted band size | 41.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SH3GL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-270 amino acids from the C-terminal region of human SH3GL1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于SH3GL1抗体的3篇参考文献示例(注：内容为虚构，仅作格式参考，实际文献需通过学术数据库检索)：
1. **文献名称**：*SH3GL1 regulates EGFR endocytosis and downstream signaling in glioblastoma cells*
**作者**：Wang L, et al.
**摘要**：该研究利用SH3GL1抗体通过免疫沉淀和免疫印迹技术，揭示了SH3GL1与表皮生长因子受体(EGFR)的相互作用，证明其通过调控EGFR内吞作用影响胶质母细胞瘤细胞的增殖和侵袭。
2. **文献名称**：*SH3GL1 modulates synaptic vesicle recycling in hippocampal neurons*
**作者**：Chen X, et al.
**摘要**：通过SH3GL1抗体的免疫荧光染色，研究者发现SH3GL1在海马神经元突触囊泡循环中起关键作用，其缺失导致突触传递效率下降，提示其在神经退行性疾病中的潜在病理机制。
3. **文献名称**：*Downregulation of SH3GL1 promotes tumor progression in hepatocellular carcinoma*
**作者**：Kim S, et al.
**摘要**：研究采用SH3GL1抗体进行组织微阵列分析，发现SH3GL1在肝细胞癌中表达显著降低，且低表达与患者预后不良相关，提示其作为肿瘤抑制因子的可能性。
建议通过PubMed、Google Scholar等平台，以“SH3GL1 antibody”或“SH3GL1 function”为关键词检索真实文献。
SH3GL1 antibody is a valuable tool for studying the SH3 domain-containing GRB2-like protein 1 (SH3GL1), a member of the endophilin family involved in membrane dynamics and intracellular trafficking. SH3GL1. also known as EEN or SH3P4. contains an N-terminal BAR domain that binds curved membranes and a C-terminal SH3 domain mediating protein-protein interactions. It plays roles in clathrin-mediated endocytosis, synaptic vesicle recycling, and actin cytoskeleton remodeling by recruiting partners like dynamin and synaptojanin. Dysregulation of SH3GL1 is linked to cancer, neurodegenerative disorders, and viral infections. For instance, reduced SH3GL1 expression correlates with tumor progression in certain cancers, while its interaction with viral proteins influences pathogen entry.
The SH3GL1 antibody detects endogenous protein levels through techniques like Western blotting, immunohistochemistry, and immunofluorescence. It aids in exploring SH3GL1’s spatial-temporal expression, post-translational modifications, and functional interactions in physiological or disease contexts. Researchers use isoform-specific antibodies to distinguish SH3GL1 from homologous family members (SH3GL2/3). Validated antibodies ensure specificity across human, mouse, and rat samples, supporting mechanistic studies on membrane trafficking pathways or therapeutic target validation. Proper controls, such as knockout cell lines, are critical to confirm antibody reliability in experimental setups.
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