| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Rap guanine nucleotide exchange factor 5, Guanine nucleotide exchange factor for Rap1, M-Ras-regulated Rap GEF, MR-GEF, Related to Epac, Repac, RAPGEF5, GFR, KIAA0277, MRGEF |
| Entrez GeneID | 9771 |
| WB Predicted band size | 67.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RAPGEF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 529-555 amino acids from the C-terminal region of human RAPGEF5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RAPGEF5抗体的3篇参考文献的简要概括(基于模拟生成，非真实文献)：
1. **文献名称**: "RAPGEF5 regulates cell adhesion and migration in cancer metastasis"
**作者**: Smith A, et al.
**摘要**: 该研究通过Western blot和免疫荧光技术，利用RAPGEF5抗体验证了其在乳腺癌细胞中的高表达，并发现其通过激活Rap1信号通路促进肿瘤细胞迁移和侵袭。
2. **文献名称**: "Role of RAPGEF5 in neuronal development and axon guidance"
**作者**: Chen L, et al.
**摘要**: 研究使用特异性RAPGEF5抗体进行组织染色，证明其在胚胎小鼠皮层神经元中表达，并通过调控细胞骨架重组影响轴突导向和突触形成。
3. **文献名称**: "RAPGEF5 deficiency disrupts vascular endothelial barrier function"
**作者**: Tanaka K, et al.
**摘要**: 通过免疫沉淀结合RAPGEF5抗体，揭示了该蛋白在内皮细胞屏障稳定性中的作用，其缺失导致VE-cadherin磷酸化异常和血管通透性增加。
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**注**：以上为模拟示例，实际文献需通过PubMed/Google Scholar检索(关键词：RAPGEF5 antibody, Rap1 signaling)获取。建议优先选择近5年、高影响因子期刊的研究。
The RAPGEF5 antibody is a research tool designed to detect and analyze the Rap Guanine Nucleotide Exchange Factor 5 (RAPGEF5), a protein encoded by the *RAPGEF5* gene in humans. Also known as MR-GEF or Repac, RAPGEF5 functions as a guanine nucleotide exchange factor (GEF) that activates Rap1. a small GTPase involved in regulating cell adhesion, proliferation, and differentiation. The protein contains conserved domains, including a catalytic CDC25 homology domain responsible for GTPase activation and a PDZ-binding motif, suggesting roles in signaling complexes and subcellular localization. RAPGEF5 is implicated in diverse physiological processes, such as neuronal development, immune response modulation, and cancer progression, where Rap1 signaling influences cell migration and metastasis.
Antibodies targeting RAPGEF5 enable researchers to investigate its expression, localization, and interactions in various tissues or cell types. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study RAPGEF5's role in signaling pathways. Dysregulation of RAPGEF5 has been associated with neurological disorders and cancers, making it a potential biomarker or therapeutic target. Validation of these antibodies often involves knockout controls or siRNA-mediated silencing to confirm specificity, as cross-reactivity with other GEFs can occur. Researchers utilize RAPGEF5 antibodies to explore its functional mechanisms in health and disease, particularly in contexts where Rap1-mediated integrin activation or cell junction dynamics are critical.
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